模拟中医病因所致脾气虚脾阳虚大鼠模型证候比较研究.docVIP

模拟中医病因所致脾气虚脾阳虚大鼠模型证候比较研究 　　 摘 要：目的：以多因素复合法分别建立脾气虚证、脾阳虚证大鼠模型，对两组模型大鼠进行比较研究以探讨脾气虚、脾阳虚的证候实质与差异。方法：动物随机分为正常组、脾气虚组和脾阳虚组，以相应造模方法进行模型复制，并以四君子汤和理中汤进行药物反证。结果：脾气虚和脾阳虚大鼠宏观证候计分高于正常组（P＜0.01），且以脾阳虚组为著（P＜0.01）；两组血清GAS均低于正常组（P＜0.01）；脾气虚组的血浆MTL低于正常组（P＜0.01），而脾阳虚组高于正常组（P＜0.01）；经健脾中药干预后，各项脾虚症状和体征逐渐缓解或消失；血清GAS、血浆MTL紊乱回复。结论：多因素复合法复制的脾气虚、脾阳虚证大鼠模型符合证候的生物学特征，稳定性良好，且经得起药物反证；脾气虚、脾阳虚大鼠的脾虚宏观证候呈递进关系；血浆MTL在脾气虚和脾阳虚中变化趋势不一致，可作为鉴别两证的微观指标。 　　 关键词：多因素；脾气虚证；脾阳虚证；大鼠模型；血清GAS；血浆MTL 　　中图分类号：R285.5 文献标识码：A 文章编号：1673-7717(2012)01-0104-04 　　Comparative Study on Model Rats with Spleen-qi Deficiency Syndrome and Spleen-yang Deficiency Syndrome 　　WANG Ying, ZHENG Xiao?wei, LI Xiu?yue 　　(Zhejiang Chinese Medical University,Hangzho 310053,Zhejiang,China) 　　Abstract:Objective: By making a comparative study on rats with spleen-qi deficiency syndrome and spleen-yang deficiency syndrome, to seek the objective property of these two syndromes and their differences. Methods: Animals were randomly divided into three groups in terms of control group, group of spleen-qi deficiency syndrome and spleen-yang deficiency syndrome. Model rats were induced by combination of multi-methods and testified with corresponding medicinals (with Sijunzi Decoction for spleen-qi deficiency syndrome and Lizhong Decoction for spleen-yang deficiency syndrome). Results: Scores of symptoms and signs in two model groups were significantly higher than those in control group (P0 01), and the difference between two model groups was significant as well, with group of spleen-yang deficiency higher (P0 01). Serum GAS was significantly decreased in model groups(P0 01). Plasma MTL was significantly decreased in group of spleen-qi deficiency (P0 01), while increased in spleen-yang deficiency (P0 01). After being treated with medicinals, the indexes mentioned above were recovered somehow. Conclusion: Multi-methods applied in this research to induce spleen-qi deficiency syndrome and spleen-yang deficiency syndrome are proved to be feasible and can b