高效液相色谱法测定藏药榜嘎中榜嘎苷A和榜嘎苷C含量.PDFVIP

906 ·药物研究· 高效液相色谱法测定藏药榜嘎中榜嘎苷A和榜嘎 苷C的含量 兰钧，旺杰次仁，阿萍 （西藏自治区食品药品检验研究院，拉萨 850000） 摘要 目的： 方法： 建立藏药榜嘎中榜嘎苷A和榜嘎苷C的HPLC含量测定方法。 采用Agilent XDB-C18 （4.6 mm×250 mm，5 μm）色谱柱，以甲醇-乙腈（9︰1）和0.4%磷酸为流动相，梯度洗脱，流速为 1.0 mL·min-1 ，检测波长为325 nm，柱温为30 ℃。结果：榜嘎苷A进样量在0.1060~6.3621 μg线性关系良好 2 2 （R=1.0000），平均回收率为98.7%；榜嘎苷C进样量在0.1059~10.5878 μg线性关系良好（R =0.9995）， 平均回收率为99.5%。结论：该方法经方法学验证，可用于藏药榜嘎的质量控制。 关键词： 榜嘎；榜嘎苷A；榜嘎苷C；高效液相色谱法；含量测定 R927.2 R28 A 1002-7777(2018)07-0906-07 中图分类号： ； 文献标识码： 文章编号： doi:10.16153/j.1002-7777.2018.07.011 Determination of the Content of Bangga Flavonoid Glycoside A and Bangga Flavonoid Glycoside C in Tibetan Herb of Bangga by HPLC Lan Jun, Wang Jieciren, A Ping (Tibet Institute for Food and Drug Control, Lhasa 850000, China) Abstract Objective: To establish an HPLC method for the determination of the content of Bangga flavonoid glycoside A and Bangga flavonoid glycoside C in Tibetan herb of Bangga. Methods: When the chromatogram column was Agilent XDB-C18 (4.6 mm×250 mm,5 µm), the mobile phase consisted of methanol-acetonitrile (9 1) ∶ and 0.4% phosphoric acid with the gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was 325 nm and the column temperature was 30 . Results: The calibration curve of Bangga flavonoid glycoside ℃ 2 A had a good linear relationship in the sample range of 0.1060-6.3621 μg (R =1.0000) and the average recovery rate was 98.7%. The calibration curve of Bangga flavonoid glycoside C also had a good linear relationship in