滇重楼连续灌胃对肠黏膜功能障碍抗炎保护作用.docVIP

滇重楼连续灌胃对肠黏膜功能障碍抗炎保护作用 　　摘要：目的 ?^察不同剂量滇重楼连续灌胃对肠黏膜功能障碍的影响，并探讨其与全身炎症反应的相关性。方法 构建大鼠肠黏膜功能障碍模型（LPS 15mg/kg），给予不同剂量滇重楼（20、40、80mg/kg）连续灌胃，于24Hr统计动物存活率及小肠推进率，取动脉全血，检测动脉血二氧化碳分压（PaCO2）、肌酸激酶（CK）、凝血酶原时间（PT）、谷草转氨酶（AST）和尿素氮（BUN）的含量，于1、7days取小肠行病理组织学检查，于1、3、7、14days取材动脉全血及小肠，ELISA法检测血浆中二胺氧化酶（DAO）及小肠中HMGB1的含量。结果 模型组大鼠与对照组比较全血中CK、PT、AST、BUN含量升高，PaCO2含量下降，存活率及小肠推进率显著下降，差异有统计学意义（P005）。1、3、7d时模型组血浆中DAO和HMGB1含量高于对照组，而1、3、7d时滇重楼治疗组低于模型组，差异有统计学意义（P005）。结论 滇重楼连续灌胃可能通过下调晚期炎症因子HMGB1表达，抑制全身炎症反应，从而改善肠黏膜屏障功能，发挥抗炎保护作用。 　　关键词：滇重楼；肠黏膜功能障碍；脂多糖；高迁移率族蛋白B1 　　中图分类号：R2855 文献标志码：A 文章编号：1007-2349（2017）10-0068-04 　　Anti-inflammatory and Protective Effects of Continuous Intestine Administration 　　of Paris Polyphylla var Yunnanensison on Intestinal Mucosal Dysfunction 　　XIONG Wei， CHEN Si-ru， XIU Guang-hui， LING Bin， SUN Jie， LIU Ping 　　（Department of Severe Cases， the Fourth Affiliated Hospital of Kunming Medical University， Kunming 650021， China） 　　【Abstract】Objective： To observe the effect of different doses of Paris polyphylla var yunnanensison on intestinal mucosal dysfunction and to explore its correlation with systemic inflammatory response Methods： The rat model of intestinal mucosal dysfunction （LPS 15mg/kg） was established and the rats were continuously administrated with different doses （20， 40， 80mg/kg） of Paris polyphylla The survival rate and the small intestine propulsion rate were measured at 24 hours The rats were sacrificed to take their arterial whole blood to determine the contents of PaCO2， creatine kinase （CK）， prothrombin time （PT）， aspartate aminotransferase （AST） and urea nitrogen （BUN） The small intestine was examined by histopathology at the 1st and 7th day The arterial whole blood and small intestine were taken to determine diamines oxidase （DAO） and HMGB1 in the small intestine by ELISA at the 1st， 3rd， 7th and 14th day Results： Compared with that of the control group， the level of CK， PT， AST and BUN in the whole blood of the model group increased， but the content of Pa