石油污染黄土土壤微生物修复实验研究.docVIP

石油污染黄土土壤微生物修复实验研究 　　摘要：为了修复西北黄土区石油污染土壤，利用优化原位土著微生物菌群辅以物理和化学相结合的修复技术，在野外调查取样的基础上，进行了土壤中石油污染的室内模拟降解实验。实验选择了不同温度（20 ℃、25 ℃、30 ℃、35 ℃），添加了氮、磷、镁、铁等元素和茅草类添加剂，适时补充氧气等。实验结果显示，土壤中石油含量在795.0 mg/kg、1 451.0 mg/kg、1 790.0 mg/kg、2 780.0 mg/kg时，经过30～45 d微生物的降解实验，土壤中石油含量降解率可达77.27%～89.06%，为黄土土壤石油污染的修复提供了技术方法和应用的可行性。 　　关键词：石油污染；微生物修复；黄土土壤 　　中图分类号：X172；X53文献标识码：A 　　文章编号：1672-1683（2008）06-0061-03 　　 　　Experimental Study on Microbial Remediation for Oil Contaminated Soil in Loess Region 　　ZHANG Sheng1，CHEN Li1，BI Er-ping2，CUI Xiao-mei3，ZHANG Cui-yun1，HAN Zhan-tao1，SHI Ying-chun1，ZHANG Fa-wang1，HE Ze1 　　(1.Institute of Hydrogeology and Environmental Geology, CAGS,Shijiazhuang 050061，China； 　　2. School of Water Resources Environment, China University of Geosciences, Beijing 100083，China； 　　3. Chinese Academy of Geological Sciences, Beijing 100037,China) 　　Abstract:In order to remediate oil contaminated soil in loess region of North Shaanxi Province, experimental studies on degradation of oil polluted soil were carried out using remediation techniques of optimized in-situ native microbial communities combined with physical chemistry methods. on the basis of field survey and sampling, the indoor simulative degradation experiments of oil polluted soil were processed, In the experiments, different temperatures (20℃, 25℃, 30℃, 35℃) were investigated, nitrogen, phosphorus, magnesium, iron and other elements, Grass-type additives and oxygen were timely added. The results of the experiment showed that degradation rate can reach 77.27%～89.06% for oil contents of 795.0 mg/kg, 1451.0mg/kg, 1790.0 mg/kg and 2780.0 mg/kg in polluted soil after 30~45 d microbial degradation. which provide methodsandfeasibility of application for microbial remediation of oil contaminated soil in loess region. 　　Key words: oil contamination；microbial remediation；soil in loess region 　　 　　1 前言 　　 　　我国西北黄土高原地区由于石油资源的大量开采利用，产生了许多环境问题。尤其是落地原油的污染已影响土壤的质量安全,土壤石油污染的防治研究工作已受到人们的重视。Jorgensen的试验显示,经生物堆埋,石油污染的土壤中