神经生长因子控释凝胶促进大鼠周围神经损伤后再生研究.docVIP

神经生长因子控释凝胶促进大鼠周围神经损伤后再生研究 　　【摘要】目的：运用纤维蛋白胶(FG)结合神经生长因子（NGF）包裹SD大鼠坐骨神经断端，通过纤维蛋白胶控释NGF以促进神经纤维再生并减少神经吻合口瘢痕形成。方法: SD大鼠24只，随机分为2组 ，每组12只。 A组：大鼠坐骨神经切断后神经外膜直接缝合，然后吻合口包裹 FG/NGF凝胶，B组：神经外膜直接缝合，吻合口未包裹凝胶。分别于术后第1、3、5、7周行术侧足迹实验检测，术后2月行术侧三头肌湿重恢复率测量、电生理测定、甲苯胺蓝染色、透射电镜分析等。结果:同一时间段两组间比较，各指标A组明显好于B组。结论：NGF缓释凝胶可以促进大鼠坐骨神经离断损伤的修复并防止吻合口与周围组织粘连。 　　【关键词】神经生长因子；纤维蛋白凝胶；周围神经；损伤；再生； 　　【Abstract】Objective：To evaluate the effect of the controlled release of biodegradable FG ,nerve growth factor (FG/ NGF) on peripheral nerve defect. Methods ：towenty-four Sprague2 Dawly rats were randomly divided into two groups, FG/ NGF(group A , n = 12),Stitching of combine (group B ,n = 12).Each rat underwent right sciatic nerve cuting into two pieces，then these defects were repaired in the two different experimental groups.The process of the peripheral nerve regeneration was evaluated at 2 months following operation,and the evaluated items were footprinting test, triceps weight ,electrophysiological ,Immunohistochemistr ,Methylene, Blue trihydrate axon morphologic assessment of the outcome of nerve regeneration. Results：Nerve regeneration in group A was comparable with that seen in natuai Nerve and was significantly superior to that in B groups in all indexes. Conclusion：: FG/ NGF controlled release was a potential ideal， that can effectively promote nerve regeneration after sciatic nerve defect of rats. The effect was as good as that of natual Nerve. 　　【key words 】 NGF; FG； Peripheral nerve； Nerve defect； Rgeneration 　　【中图分类号】R722.12 【文献标识码】B【文章编号】1004-4949(2014)06-0200-01 　　 　　材料与方法 　　1．主要试剂及设备：重组大鼠NGF及bFGF,NF200及S100兔抗鼠抗体，纤维蛋白凝胶，电生理仪及信息采集系统等。健康幼年雄性 SD 鼠 24 只，体重 250～300 g ,随机分为 A（实验组）、 B (对照组）两组 ,每组12 只；该课题研究所用动物（SD鼠）、全部的手术流程，以及SD鼠的全部喂养流程全部符合1996年版《北京市实验动物管理条例》的相关规定。 　　2．动物实验模型制作：所有大鼠的手术部位为右大腿 ,左大腿设为正常对照。术区常规备皮，10%水合氯醛腹腔注射麻醉，于大鼠右股后外侧行纵形切口,显露坐骨神经约2. 5 cm。A 组于右侧坐骨神经上中段横行切断后以11-0无创显微缝合线外膜缝合法连续缝合4针，将配置好的FG/ NGF取适量涂抹于吻合神经断端，最后将肌肉和皮肤逐层缝合，并在切口处涂抹少许青霉素粉末。B组手术方法同A组，但神经吻合断端只行外膜缝合，未予FG/ NGF涂抹。术后8周行足迹试验、神经电生理测定、