肝细胞癌患者血清miR―155表达及临床意义.docVIP

肝细胞癌患者血清miR―155表达及临床意义 　　[摘要] 目的 研究肝?胞癌（HCC）患者血清中microRNA-155（miR-155）的表达水平及其临床意义。 方法 采用实时荧光定量PCR的方法检测50例HCC患者的术前血清及50例健康人血清的miR-155相对表达量，分析血清miR-155表达水平与临床病理参数及术后1年无复发生存（RFS）率的关系。 结果 31例（62%）HCC患者血清miR-155相对表达量高于健康人血清（P=0.03），血清miR-155表达与HCC癌组织的Edmonson分级（P=0.01）及脉管侵犯（P=0.02）相关，而与年龄、性别、肿瘤大小、肿瘤个数、肝硬化、分期、HBV DNA拷贝数及AFP均无关（P0.05）。单因素分析显示脉管侵犯及肝硬化与术后1年RFS率低正相关。血清miR-155高表达者比低表达者1年RFS率低（45.9% vs76.6%），差异有统计学意义（P=0.02）。多因素分析显示脉管侵犯（P=0.15）及miR-155（P=0.05）是影响HCC患者术后1年RFS率的独立危险因素。 结论 血清miR-155是一个对HCC恶性程度的判断、侵袭性生物学行为的预测和术后预后评估都很有价值的生物指标。 　　[关键词] miR-155；肝细胞癌；血清；预后 　　[中图分类号] R735.7 [文献标识码] A [文章编号] 2095-0616（2017）11-09-06 　　Expression of serum miR-155 of patients with hepatocellular carcinoma and its clinical significance 　　LI Tuhua1 GUAN Chengnong2 LI Yue3 　　1.Center of Oncology，The Affiliated Hospital of Guangdong Medical University，Zhanjiang 524001，China；2.The Second Clinical Medical College，Guangdong Medical University，Dongguan 523808，China；3.Graduate School，Guangdong Medical University，Zhanjiang 524001，China 　　[Abstract] Objective To research the expression level of serum microRNA-155 （miR-155） of patients with hepatocellular carcinoma （HCC） and its clinical significance. Methods Preoperative serum of 50 patients with HCC and relative expression of serum miR-155 of 50 healthy cases were detected by real-time fluorescence quantitative PCR.Relationships between expression level of serum miR-155 and clinical pathological parameters and rate of recurrence-free survival （RFS） of one year after operation were analyzed. Results Relative expression of serum miR-155 of 31 patients （62%） with HCC was higher than that of healthy cases（P=0.03）. Expression of serum miR-155 was related to Edmonson grading of HCC（P=0.01） and vessel invasion（P=0.02）.But it had no relation with age，sex，size of tumors，number of tumors，cirrhosis，staging，HBV DNA copy number and AFP （P0.05）.Univariate analysis showed that vascular invasion and liver cirrhosis was positively correlated with RFS rate of