羟基喜树碱对人乳腺癌MCF7细胞增殖及凋亡影响.docVIP

羟基喜树碱对人乳腺癌MCF7细胞增殖及凋亡影响 　　[摘要] 目的：探讨羟基喜树碱在体外对人乳腺癌MCF-7细胞增殖及凋亡的影响，为乳腺癌的临床用药提供实验依据。方法：将不同浓度（10 μmol/L、50 μmol/L、100 μmol/L、200 μmol/L）的羟基喜树碱作用于人乳腺癌MCF-7细胞48 h，并设不加羟基喜树碱溶液的MCF-7细胞作为对照组，用MTT法测定MCF-7细胞的增殖情况，用Hoechst33258染色检测MCF-7细胞的凋亡情况，以RT-PCR检测羟基喜树碱对MCF-7细胞Casepase-3 mRNA表达的影响。结果：与对照组比较，50 μmol/L、100 μmol/L及200 μmol/L羟基喜树碱对细胞增殖均有抑制作用（P＜0.05、P＜0.01），尤以200 μmol/L的抑制作用最明显，其抑制率呈浓度依赖性增加。荧光显微镜观察显示，100 μmol/L及200 μmol/L羟基喜树碱处理组中可见典型的凋亡形态学改变。与对照组比较，各羟基喜树碱处理组中MCF-7细胞Caspase-3 mRNA表达水平均升高（P＜0.05、P＜0.01），并呈浓度依赖性升高趋势。结论：羟基喜树碱可在体外抑制MCF-7细胞增殖，并通过Caspase-3途径诱导细胞凋亡，达到抑制肿瘤细胞生长的作用。 　　[关键词] 羟基喜树碱；增殖；凋亡；乳腺癌 　　[中图分类号] R737.9 [文献标识码]A [文章编号]1673-7210（2011）04（a）-017-03 　　 　　Effect of Hydroxycamptothecin on the proliferation and apoptosis of human breast cancer cell line MCF-7 　　CHEN Fei1, WANG Yumei2 　　1.The Hospital of Yibin College, Sichuan Province，Yibin 644000, China; 2.Department of Gastroenterology, the First People′s Hospital of Yibin City, Sichuan Province, Yibin 644000, China 　　[Abstract] Objective: To explore the effect of Hydroxycamptothecin (HCPT) on the proliferation and apoptosis of human breast cancer cell line MCF-7 in vitro, and to provide experimental basis for the clinical medication of breast cancer. Methods: HCPT with different concentrations (10 μmol/L, 50 μmol/L, 100 μmol/L, 200 μmol/L) were used to treat MCF-7 cells for 48 hours, and MCF-7 cells without HCPT were served as the control group. MTT assay was applied to determine the proliferation of MCF-7 cells, Hoechst33258 staining was used to detect the apoptosis of MCF-7 cells, and RT-PCR was used to detect the effect of HCPT on the expression of Casepase-3 mRNA in MCF-7 cells. Results: Compared with the control group, 50 μmol/L, 100 μmol/L and 200 μmol/L HCPT showed obvious inhibitory effect on MCF-7 cells (P＜0.05, P＜0.01), and the inhibitory effect of 200 μmol/L was the most obvious, the inhibitory rate showed a concentration-dependent tendency. Fluorescence microscope observation showed that typical apopt