量子点标记免疫层析试纸条快速检测莱克多巴胺研究.PDFVIP

254 2009, Vol. 30, No. 12 食品科学 ※分析检测 量子点标记免疫层析试纸条快速检测 莱克多巴胺的研究 张国华，赖卫华，熊勇华，陈 媛 (南昌大学 食品科学与技术国家重点实验室，江西 南昌 330047) 摘 要：本实验通过莱克多巴胺抗原抗体的制备、量子点的制备、试纸条的组装和测试等步骤研究一种将量子点 应用于莱克多巴胺免疫层析试纸条的新方法。结果表明，莱克多巴胺快速检测试纸条的检测限为 3ng/ml ，检测时 间为 10min 。本法使用方便、经济，适合于莱克多巴胺现场快速检测。 关键词：莱克多巴胺；量子点；免疫层析试纸条 Application of Quantum Dot Labeling to Development of Immunochromatographic Test Strip for Rapid Detection of Ractopamine ZHANG Guo-hua ，LAI Wei-hua ，XIONG Yong-hua ，CHEN Yuan (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China) Abstract ：The quantum dot labeling technique was applied to the development of immunochromatographic test strip for rapid detection of ractopamine. Four steps were involved in the procedure of preparation of the strip, including the preparation of antigen and antibody against ractopamine, the production of quantum dots, and the installation and testing of the strip. The detection limit for ractopamine by this strip was 3 ng/ml, and the detection could be finished within 10 min. Apart from being time-saving, the strip is easy to perform and relatively inexpensive. It is therefore ideally suited for on-site testing of ractopamine. Key words ：ractopamine ；quantum dots ；immunochromatographic test strip 中图分类号：TS207.7 文献标识码：A 文章编号：1002-6630(2009)12-0254-04 莱克多巴胺盐酸盐(简称 RAC) ，是一种苯酚胺类 β- 用于生物分子标记的半导体纳米粒子连接到生物分 肾上腺素激动剂，能选择性激动支气管平滑肌的 β- 受 子上时，必须是水溶性的，这可通过在其表面引入极 体。动物实验表明，当用药量为治疗剂量的 5 ～1 0 倍 性的官能团达到，实现量子点表面连接亲水性的官能团 时，可促使多种动物体内的营养成分由脂肪向肌肉转 再与生物材料相连可利用量子点表面的元素如 Zn 、Cd [ 1 -3 ] 等与巯基之间强的络合作用力，使量子点与巯基酸络 移，表现为营养“再分配效应”