膈下逐瘀汤对非酒精性脂肪肝大鼠脂质代谢和血小板活化影响.docVIP

膈下逐瘀汤对非酒精性脂肪肝大鼠脂质代谢和血小板活化影响 　　[摘要] 目的 研究膈下逐瘀??对非酒精性脂肪肝（NAFLD）大鼠的治疗作用及机制。 方法 雄性SD大鼠按完全随机分组原则分为空白组、模型组和药物干预组。空白组基础饲料喂养，其余高脂饲料喂养，持续喂养12周。8周末药物干预组开始给药，动态观察各组大鼠肝组织病理变化，检测各组大鼠肝指数、血清总胆固醇（TC）、三酰甘油（TG）、丙氨酸氨基转移酶（ALT），肝组织TG和TC含量，以及血小板聚集率和可溶性P-选择素（CD62p）的含量。 结果 8周末模型组HE染色的肝组织病理切片观察到脂肪变性的肝细胞占肝小叶的比例1/3，证明造模成功，12周末模型组大鼠各项检测指标与空白组比较含量均有不同程度升高，差异有统计学意义（P 0.05或P 0.01）；与模型组比较，药物干预组大鼠各项检测指标含量均有不同程度降低，差异有统计学意义（P 0.05或P 0.01）。 结论 膈下逐瘀汤对于改善NAFLD大鼠肝细胞脂质沉积有着显著的疗效，作用机制可能与调节脂质代谢、减少CD62p的表达、降低血小板聚集率有关。 　　[关键词] 膈下逐瘀汤；非酒精性脂肪肝；血小板；可溶性P-选择素 　　[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210（2016）11（c）-0004-04 　　Effects of Gexia Zhuyu Decoction on lipid metabolism and platelet activation in rats with non-alcoholic fatty liver disease 　　ZHU Xiaosong FANG Tao CHEN Wenhui TAN Liping SHI Anhua 　　Basic Medical College， Yunnan University of Traditional Chinese Medicine， Yunnan Province， Kunming 650500， China 　　[Abstract] Objective To study the effects and mechanism of Gexia Zhuyu Decoction in rats with non-alcoholic fatty liver disease （NAFLD）. Methods Male Sprague Dawley （SD） rats were randomized completely into three groups： blank group， model group and drug group. Blank group was fed with common diet， the others were fed with high-fat diet for 12 weeks. At the 8th weekend， the drug group was given Gexia Zhuyu Decoction. The pathological changes of hepatic tissue in rats of all groups were observed dynamically. The liver index， total cholesterol （TC）， triglyceride （TG）， alanine aminotransferase （ALT） in serum， TC， TG in liver specimens， and platelet aggregation， soluble P-selectin （CD62p） were measured. Results At the end of 8th weekend， the pathological section of hepatic tissue by HE staining in model group showed that， the proportion of hepatic cells with fatty degeneration accounted for hepatic lobule was over 1/3， which proved that， the model was successful. At the end of 12th weekend， all indexes of model group were higher than those in blank group， the differences were statistically signifi