胞质m-csf对人乳腺癌mcf-7细胞抗肿瘤药物抗性的影响肿瘤学专业论文.docxVIP

 PAGE 5 性，降低细胞内阿霉素和紫杉醇的浓度；胞质 M-CSF 上调 MCF-7 细胞 MDR1 基因、P-gp 和 Bcl-2 蛋白的表达。 关键词： M-CSF；MCF-7 细胞；抗肿瘤药物；多药耐药。 EFFECTS OF CYTOPLASMIC M-CSF ON THE SENSITIVITY OF ANTI-TUMOR CHEMOTHERAPEUTIC DRUG IN HUMAN BREAST CANCER ABSTRACT OBJECTIVES: To explore mechanism and effects of cytoplasmic M-CSF on the sensitivity of anti-tumor chemotherapeutic drug in human breast cancer cells. METHODS: The effect of cytoplasmic M-CSF on the sensitivity of anti-tumor chemotherapeutic drug of MCF-7 cells was analyzed by MTT and trypan blue staining. The concentration of adriamycin(ADM) and paclitaxel(TAX)within MCF-7 cells was measured by high efficiency fluid chromatography(HPLC). The expression of P-gp and Bcl-2 protein was detected by Western blot. The expression of MDR1 gene was assayed by RT-PCR RESULTS: The results from MTT and trypan blue staining indicated that the relative survival rate of pCMV/cyto/myc-M-CSF-transfected MCF-7 cells(referred as to MCF-7-M cell) is higher than either pCMV/cyto/myc transfected MCF-7 cells (referred as to MCF-7-C cell) or untransfected MCF-7 cells (referred as to MCF-7 cell), There was no significant difference between that of MCF-7-C cells and MCF-7 cells(p＞0.05).The results from HPLC showed that there was less ADM and TAX within MCF-7-M cells than either MCF-7-C and MCF-7 cell line(p＜0.01). Our results indicated that MCF-7-M cell had a higher expresion of MDR1gene , Bcl-2 and P-gp protein , compared with MCF-7-C and MCF-7 cell line(p＜0.01). CONCLUSION: Cytoplasmic M-CSF enhances the resistance of MCF-7 cell to ADM and TAX and reduces the concentration of these two drugs within MCF-7 cell. Cytoplasmic M-CSF up-regulates the expression of Bcl-2 and P-gp protein and MDR gene in MCF-7 cells. Li Ni (Department of Oncology) Directed by Professor Jiang Hao KEY WORDS: Macrophage colony-stimulating factor (M-CSF); MCF-7 cell line; Chemotherapeutics; Multidrugresistance. 6 前 言 乳腺癌是女性最常见的恶性肿瘤之一。在西方国家，其发病率居女性恶性肿 瘤的第一位；在我国乳腺癌的发病率近年来迅速上