血清脂联素在肥胖哮喘小鼠体内变化及其作用.docVIP

血清脂联素在肥胖哮喘小鼠体内变化及其作用 　　【摘要】目的:检测肥胖哮喘小鼠血清脂联素水平，探讨肥胖对哮喘慢性气道炎症和气道重塑的影响及其机制。方法:45只雌性C57/6J小鼠随机分为对照组（C组）、单纯肥胖组（B组）、肥胖哮喘组（A组）。统计各组小鼠体重、支气管肺泡灌洗液（BALF）中细胞总数及分类、肺组织病理切片观察气道重塑程度及ELISA法测定血清脂联素的水平。结果: A组小鼠BALF中白细胞总数及嗜酸性粒细胞比例较B组、C组明显增高（P0.05）；小鼠血清脂联素水平按C组、B组、A组顺序依次降低（P0.05）。Pearson相关分析表明, 小鼠气道管壁厚度(WAt/Pbm)、平滑肌厚度(WAm/Pbm)与脂联素水平呈负相关（P0.05）。结论:肥胖加重了哮喘小鼠气道炎症与气道重塑，脂联素水平失衡可能是其加重的重要因素。 　　【关键词】哮喘；肥胖；脂联素 　　The effect of serum adiponectin in obesity asthma mice 　　Shujuan Lan Guoyu Man Yongchun Li Wei Han 　　【Abstract】 Objective To investigate the serum adiponectin in obese asthmatic mouses, and evaluate the effects of obesity on the airway inflammation and remodeling of chronic asthma.MathodsForty-five female C57BL/6J mice were randomly divided into obese asthma group (A group). obesity group (B group) and normal control group (C group), The cells in BALF counted and classified after staining, the pathological changes of lungs and the serum adiponectin and leptindetected by ELISA were compared between 4 groups.ResultsThe BALF cells in A group are much higher than that in C group and B group(P0.05). Serum adiponectin was decreased in the turn of C group, B group and A group (P0.05). Pearson correlation analysis showed that the airway wall (WAt/Pbm) or smooth musle (WAm/Pbm) were in negative correlation with adiponectin(P0.05).Conclusions Obese increased the airway inflammation and remodeling of chronic asthma, theinbalance of leptin and adiponectin levels may be an important factor. 　　【Key words】Asthma；obesity；adiponectin 　　【中图分类号】R372 【文献标识码】B【文章编号】1005-0515(2011)08-0004-03 　　肥胖已成为支气管哮喘独立的危险因素，肥胖和超重可增加哮喘发生的危险性，其在哮喘的发生、发展中的作用日益被人们所关注。肥胖和哮喘之间关联的基础可能与慢性全身性炎症以及能量调节激素等有关［1］。瘦素（leptin）和脂联素（adiponectin）是脂肪细胞分泌诸多细胞因子中最主要的两个脂肪因子。本研究在营养性肥胖哮喘小鼠模型上，通过检测小鼠体内血清脂联素水平，探讨其在哮喘和肥胖哮喘体内变化及其对气道炎症及气道重塑的作用。 　　1 材料与方法 　　1.1 材料:3-4周龄C57/6J雌性健康小鼠45只，体重8-10g，清洁级，购自中国军事医学科学院实验动物中心；卵白蛋白干粉（ovalbumin，OVA，GradeV）购自美国SIGMA公司；佐剂液态铝［Imject Alum(AI(OH)3/Mg(OH)2］购自美国PIERCE公司；胆固醇购自上海生物工程技术有限公司；脂联素ELISA检测试剂盒购自美国RD公司。