血管性痴呆与神经束蛋白155相关性研究.docVIP

血管性痴呆与神经束蛋白155相关性研究 　　【摘要】 目的：探讨慢性脑缺血致血管性痴呆与神经束蛋白155的相关性。方法：2013年12月-2014年5月期间选用Wistar大鼠40只，随机分为模型组24只、对照组12只。模型组大鼠使用双侧颈总动脉永久结扎法（2VO）制备慢性前脑缺血致血管性痴呆动物模型，对照组仅手术而不行双侧颈总动脉结扎。使用Morris水迷宫对各组大鼠手术前和手术后的记忆行为数据进行检测。比较手术前后及各组间逃避潜伏期的差异，明确模型组大鼠是否达到痴呆。术后30 d和60 d取大鼠脑组织并制成匀浆，使用ELISA检测各组NF155的表达情况。结果：造模前模型组小鼠逃避潜伏期与假手术对照组比较差异无统计学意义（P0.05）。造模后，术后30 d组第6天开始，逃避潜伏期均明显长于假手术对照组，差异均有统计学意义（t=2.667、6.812、8.743、10.775；P=0.014、0.000、0.000、0.000）；术后60 d组第5天开始，逃避潜伏期均明显长于假手术对照组，差异均有统计学意义（t=3.016、4.137、7.562、7.910、12.686；P=0.006、0.000、0.000、0.000、0.000）。经ELISA检测显示模型30 d组及60 d组大鼠NF155浓度分别为（48.27±7.32）、（37.65±6.88）ng/mL，与假手术对照组（85.07±6.75）ng/mL比较，差异均有统计学意义（P0.05），说明模型组大鼠脑内NF155的含量较对照组明显下降。结论：脑内NF155含量的变化有可能参与了慢性缺血所致血管性痴呆的发生。 　　【关键词】 慢性脑缺血； 血管性痴呆； 神经束蛋白155 　　The Correlation Study between Vascular Dementia and NF155/ZHAO Teng，CHEN Hai-long，LIU Jing-yao，et al.//Medical Innovation of China，2015，12（30）：014-016 　　【Abstract】 Objective：To investigate the correlation between vascular dementia by chronic ischemia and NF155. Method：40 male wistar rats were randomly divided into model group of 24 cases and the control group of 12 cases from December 2013 to May 2014.Rats in the model group were treated with permanent bilateral carotid artery ligation（2VO） to prepare chronic forebrain ischemia induced animal model of vascular dementia， and the rats in the control group just treated surgery.Morris water maze was given in each group before and after operation to measure the data of memory behavior，than compared the different escape latency between groups before and after operation to make it clear whether the rats in model group dementia.The brain of rats were made into homogenate， than ELISA was given to measure the NF155 expression of every group. Result：There were no significant differences between the model group and the sham control group before modeling（P0.05）.After modeling， the escape latency of 30 days after surgery group were significantly longer than the sham control group in the sixth day（t=2.667，