长柱重楼中主要甾体皂苷类成分定性定量分析.docVIP

长柱重楼中主要甾体皂苷类成分定性定量分析 　　[摘要]为了从化学成分上研究长柱重楼是否具有重楼药材代用品的开发价值，该研究利用UPLCQTOF MS比较了长柱重楼和滇重楼的化学成分，并进一步利用HPLCUV同时测定了77株不同来源的长柱重楼中8 种重楼皂苷的含量。结果显示长柱重楼和滇重楼的化学成分相似，长柱重楼化学成分更为复杂，鉴定的22个主要共有峰均为甾体类化合物。不同株长柱重楼中重楼皂苷的含量具有一定的差异，其中药典规定4种甾体皂苷（重楼皂苷Ⅰ，Ⅱ，Ⅵ，Ⅶ）总量最高的为330%，最低的为0068%，8种甾体皂苷总量最高的为618%，最低的为071%。长柱重楼中78%的样品符合《中国药典》要求，整体质量较为稳定，可以作为重楼替代品种进行研究开发。 　　[关键词]重楼； 长柱重楼； 甾体皂苷； 定性定量分析 　　Qualitative and quantitative analyses of primary saponins in Paris forrestii 　　HUANG Yuanyuan1，2， KANG Liping1， PENG Huasheng2， LIU Dahui3， HAO Qingxiu1， 　　ZHAO Jialiu4， CHEN Min1*， HUANG Luqi1* 　　（1 State Key Laboratory Breeding Base of Daodi Herbs， National Resource Center for Chinese Materia Medica， 　　China Academy of Chinese Medical Sciences， Beijing 100700， China； 　　2Anhui University of Chinese Medicine， Hefei 230012， China； 　　3College of Pharmacy， Hubei University of Chinese Medicine， Wuhan 430065， China； 　　4Lanping County in Ecological Industry Co.， Ltd， Nujiang 673100， China） 　　[Abstract]In order to study whether Paris forrestii could be developed as a substitute of Paridis Rhizome， chemical compositions of P. forrestii and P. polyphylla var yunnanensis were investigated by UPLCQTOF MS In addition， the contents of eight primary steroidal saponins in 77 batches of P. forrestii samples from different habitats were simultaneously determined by HPLCUV The results showed that P. forrestii and P. polyphylla var yunnanensis have similar chemical compositions， and all 22 major common peaks were identified as steroid derivatives Meanwhile， there were some differences in the contents of saponins in P. forrestii samples from different habitats The contents of 4 steroidal saponins in Chinese Pharmacopoeia ranged from 0068% to 330%， and the highest content of the 8 kinds of steroidal saponins was 618%， while the lowest was just 071% Moreover， 78% of P. forrestii samples were in conformity with the requirements of Chinese Pharmacopoeia， indicating that P. forrestii samples had relatively stable quality and