陈皮利咽合剂对小鼠气管分泌物影响研究.docVIP

陈皮利咽合剂对小鼠气管分泌物影响研究 　　[摘要] 目的 研究陈皮利咽合剂对小鼠气管分泌物的影响，以期探讨其祛痰作用及对急慢性咽炎的治疗作用。 方法 50只小鼠随机分为生理盐水组、银黄片混悬液组和陈皮利咽大、中、小剂量组，每组各10只，连续灌胃给药5 d，于末次给药后1 h，腹腔注射酚红溶液，处死小鼠，取下相同位置的一段气管并冲洗气管，测洗出液的吸光度，计算气管冲洗液中酚红浓度。 结果 陈皮利咽合剂可显著促进小鼠气管的分泌，显著增高各剂量组气管洗出液的吸光度。 结论 陈皮利咽合剂有很好的祛痰作用。 　　[关键词] 陈皮利咽合剂；祛痰；急慢性咽炎 　　[中图分类号] R285.5 [文献标识码] A [文章编号] 1674-4721（2013）11（a）-0115-02 　　Study on influence of Chenpi liyan mixture on tracheal secretions of mice 　　WANG Ying LI Xiang-yu XIE Xin-ping LOU Dan 　　The First Affiliated Hospital of Henan University of Science and Technology，Luoyang 471003，China 　　[Abstract] Objective To study of influence of Chenpi liyan mixture on tracheal secretions of mice，to explore its expectorant action and therapeutic effect of Chenpiliyan mixture for acute and chronic pharyngitis. Methods 50 mice were randomly assigned into the normal saline group，Yinhuang tablet suspension group and large，medium and small dose of Chenpiliyan mixture group，there were 10 mice in each group respectively，all mice were given continuous intragastric administration for 5 days and were executed by intraperitoneal injection of phenol red solution at the time of one hour after the last administration，the trachea segments in the same position were resected and flushed，the absorbances and concentration of phenol red of the eluate were measured and calculated. Results Chenpiliyan mixture can significantly promote the secretion of trachea in mice，and notably increase the absorbances of the trachea eluate in groups of all dose. Conclusion Chenpiliyan mixture has good expectorant action. 　　[Key words] Chenpi liyan mixture；Expectorant；Acute and chronic pharyngitis 　　咽炎属祖国医学的“喉痹”范畴，现代医学将其分为急性和慢性两大类，认为急性咽炎是咽黏膜、黏膜下组织和淋巴组织的急性炎症，病因以病毒和细菌感染较多见，慢性咽炎多由急性咽炎反复发作转为慢性，或因鼻病长期张口呼吸刺激咽部，或因长期吸烟饮酒、过食辛辣及粉尘、化学气体等因素造成咽黏膜、黏膜下组织和淋巴组织的慢性炎症。陈皮利咽合剂是本院多年来治疗急慢性咽炎的经验方，具有行气解郁、燥湿化痰、养阴利咽、活血止痛的功效，并取得较好的临床疗效，为了探讨其作用机制，本院进行以下实验研究。 　　1 材料与方法 　　1.1 药物试剂 　　陈皮利咽合剂，由河南科技大学第一附属医院生产，批号为090305，用法用量：口服，20～30 ml/次，3次/d；银黄片，由哈药集团三精黑河药业有限公司生产，批号为080