应用DGGE技术研究玉米储藏过程中的霉菌群落时空分布特征.PDFVIP

※安全检测 食品科学 2018, Vol.39, No.04 307 应用DGGE技术研究玉米储藏过程中的 霉菌群落时空分布特征 岳晓禹，张 华，陈威风，邹 建，李 欣，杨 娜 （河南牧业经济学院食品工程学院，河南郑州 450046 ） 摘  要：研究储粮过程中霉菌污染的发生规律，旨在减少霉菌及真菌毒素的污染，提高我国粮食安全。利用变性梯 度凝胶电泳研究粮库中不同储藏时间和空间的玉米样品中霉菌群落的特点。结果表明，本研究中储藏玉米的霉菌污 染以青霉、曲霉和毛霉为主，其霉菌群落的变化与储藏时间具有较强的相关性，而与其在粮库中的空间位置相关性 1 a 3 a 2 a 较小。在储藏时间方面，储藏 和 的样品中霉菌群落具有较大的差异，而储藏 样品的霉菌群落处于过渡期状 态。本实验探究储藏玉米中霉菌群落的时空分布特征，可以为建立准确可靠的霉菌群落模型提供理论支持和数据支 持。 关键词：玉米；霉菌；变性梯度凝胶电泳 Temporal and Spatial Distribution Characteristics of Mold Communities during Storage of Corn as Studied by Denaturing Gradient Gel Electrophoresis YUE Xiaoyu, ZHANG Hua, CHEN Weifeng, ZOU Jian, LI Xin, YANG Na (College of Food Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China) Abstract: In this paper, denaturing gradient gel electrophoresis (DGGE) was used to research the community composition of molds in corn grains stored for different years at different spatial locations in the grain depot. The aim of this investigation was to understand the pattern of mold contamination during grain storage for the purpose of reducing mold and mycotoxin Penicillium Aspergillus Mucor contamination and improving the safety of grains. The results revealed that , and were the main molds found in stored corn. The mold community composition was closely correlated with storage duration but less correlated with spatial location in the depot. The corn samples stored for 1 year and 3 years showed great differences in mold