高压培养对血管内皮细胞tPA和PAI1影响及卡托普利干预效果.docVIP

高压培养对血管内皮细胞tPA和PAI1影响及卡托普利干预效果 　　[摘要]目的：高血压常伴有纤溶功能的异常，但其机制尚不清楚。本研究拟观察高静水压培养对人脐静脉内皮细胞（HUVECs）t-PA和PAI-1的影响以及卡托普利的干预效果，并探讨其可能的作用机制。方法：选用第4～6代HUVECs，接种于24孔培养板中。依培养压力分为3组：大气压组（0 mmHg），中压组（90 mmHg），高压组（180 mmHg）。在同一压力组，根据不同药物干预又分为两个亚组。即对照组（Ctrl）和卡托普利组（Cap，10-5 mol/L）。每组6份标本。采用ELISA法测定上清液t-PA和PAI-1的抗原浓度，并用细胞内总蛋白进行标化（单位：ng/μg proteins）。同时测定细胞内Ca2+浓度（nmol/L）。结果：与大气压组相比，中压和高压组t-PA浓度均显著降低，PAI-1浓度显著增高，t-PA/PAI-1比值显著降低，[Ca2+]i也显著增高。卡托普利对大气压组的t-PA、PAI-1和[Ca2+]i无显著影响，但在两个高压组，卡托???利显著升高t-PA浓度，显著降低PAI-1浓度，t-PA/PAI-1比值显著升高，[Ca2+]i显著地降低。结论：高静水压可损害内皮细胞的纤溶功能，而卡托普利的干预可降低高压所升高的[Ca2+]i，并改善高静水压对内皮细胞纤溶功能的影响。 　　[关键词]血管内皮细胞；高静水压；卡托普利；组织型纤溶酶原激活物；纤溶酶原激活物抑制剂-1 　　[中图分类号]R34 [文献标识码]B[文章编号]1673-7210（2007）10（c）-077-03 　　 　　Effects of high hydrostatic pressure on the t-PA and PAI-1 secretions of vascular endothelial cells and the interfering results of Captopril 　　LU Yin-hui1,CHENG Xiao-shu2, SU Hai2, RAO Fang2 　　(1.Deartment of Officers Health, Jiangxi Provincial Peoples Hospital, Nanchang 330006,China;2.Department of Cardiology, The Second Affiliate Hospital of Jiangxi Medical College,Nanchang 330006,China) 　　[Abstract]Objective: Clinical studies have demonstrated that hypertension often accompanies abnormality of fibrinolytic function. In this study,we investigated the effects of high hydrostatic pressure on the concentrations of t-PA and PAI-1 antigen in culture medium of human umbilical vein endothelial cells(HUVECs) and observed the interfering results of Captopril. Methods: HUVECs harvested between passages 4 and 6 were used for the experiments.According to the cultured pressure, they were divided into three groups:atmospheric pressure group(0 mmHg),middle pressure group(90 mmHg),and high pressure group(180 mmHg). The above three groups were respectively divided into two subgroups（6 samples/subgroups）, control group and Captopril group(10-5 mol/L). At the end of the culture period, supernatants were collected to measure the concentrations of t-PA and PAI-1 antigens by using ELISA kit.