油茶根际溶磷菌3-Y-08筛选和鉴定.PDFVIP

东农业科学 2015 年第 3 期 47 油茶根际溶磷菌3-Y-08 的筛选与鉴定 刘小玉 付登强 陈良秋 杨伟波 李东霞 符海泉 贾效成 中国热带农业科学院椰子研究所/油茶研究中心袁 海南 文昌 571339冤 摘要 采用传统的微生物分离培养法袁对油茶根际溶磷菌进行分离袁筛选出6 株溶磷细菌遥 利用透明圈法 对油茶根际土壤中具有溶磷能力的细菌进行初筛曰采用钼锑抗比色法测定发酵液的可溶性磷含量袁对解磷菌 株进行复筛袁得出菌株 3-Y -08 的溶磷活性最强遥 根据进行菌落形态特征尧生理生化特征尧16S rDNA 序列和系 统发育分析等研究袁初步鉴定菌株 3-Y -08 为巨大芽孢杆菌遥 关键词 油茶曰 溶磷菌曰 筛选曰 鉴定 中图分类号 S154.39 文献标识码 A 文章编号 1004-874X 2015冤03-0047-04 Screening and identification of phosphate solubilizing bacteria in the rhizosphere of camellia LIU Xiao-yu 袁FU Deng-qiang 袁CHEN Liang-qiu 袁YANG Wei-bo 袁 LI Dong-xia袁FU Hai-quan 袁JIA Xiao-cheng (Coconut Research Institute/ Oil Came llia Researc h Cente r, CA TA S, W enc hang 571339 袁 China) A bstract: Phosphate solubilizing bacteria were isolated from the rhizosphere of camellia 袁and six strains were screened by using traditional method of isolation and culture of microorganisms. By using the way of clear zone, phosphate solubilizing bacteria from the rhizosphere soil were preliminarily isolated, and the high effective phosphate solubilizing bacteria were further screened by using moly bdenum-antimony anti-spectrophotometric method, strain 3- Y -08 was the most active. The strains and 16S rDNA and phy logenetic tree were identified by study ing the morphology, cultural, physiological and biochemical properties. Thus the strain was preliminarily identified as Bac illus me gate rium. Key words: camellia; phosphate solubilizing bacteria; screening; identification [3] [