牦牛DAZL基因编码区的克隆和序列特征与进化分析.PDF

2010, 33 ( 3): 109- 114 Journal of N anj ing A gricultural University http: //nauxb1njau1 edu1 cn , , , . DAZL [ J] 1 , 2010, 33 ( 3): 109- 114 DAZL 1 2 1 3 1 1 1 1* , , , , , , , ( 11 , 21 , 210095; 31 , 100193) : DAZL , PCR DAZL , DA ZL : DAZL cDNA 1 782 bp, 885 bp, 295, 981 31% ; DAZL DAZ RNADAZ : , , : ; DA ZL ; ; ; + : S82318 5 : A : 1000- 2030 ( 2010) 03- 0109- 06 Cloning and characterization of coding region ofDA ZL gene and evolution analysis in yak 1 2 1 3 1 LIX in-fu, X IE Yuan-cheng, ZHANG Qing-bo , ZHAO X ing-bo , GU Yao , 1 1 1* ZHU X iang, X IE Zhuang, L IQi-fa ( 11 College ofAnmi alSciences andTechnology, 21College of Information Sciences and Technology, Nanjing AgriculturalUniversity, Nanjing 210095, China; 31State Key Laboratory for Agribiotechnology, China AgriculturalUniversity, eijing 100193, China) Abstract: ased on the Deleted in Azoosperm ia-L ike (DAZL ) gene sequences of cattle, prmi ers were designed to amplifyDA ZL gene coding region using PCR in testis ofyak. The sequence structure of theDAZL gene coding region and the phylogenetic relation- ship w ith other species were analyzed by application of the bioinformatics sofwt are. The PCR amplification results show ed that the sequenceswere 1 782 base pairs long and the encoding region contained 885 bp encoding