高迁移率蛋白A2在睾丸肿瘤组织中表达及临床意义.docVIP

高迁移率蛋白A2在睾丸肿瘤组织中表达及临床意义 　　【摘要】目的：探讨HMGA2在睾丸癌组织中的表达及意义。方法：运用荧光定量PCR（Q-PCR）和Western blot 方法检测HMGA2mRNA和蛋白在17例睾丸肿瘤和15例正常睾丸组织中的表达差异、免疫组织化学（IHC）方法检测HMGA2蛋白在44例睾丸肿瘤，30例良性组织芯片中的表达差异。结果：HMGA2mRNA表达在癌组织中明显高于良性组织：（9.09±2.95） vs. （3.86±1.66）， P=0.036、HMGA2蛋白在癌肿组织中的表达亦显著高于良性组织：（9.27±2.91） vs. （4.66±1.62）， P=0.037。 HMGA2蛋白的表达高低和睾丸肿瘤的临床分期及肿瘤大小密切相关，P0.05。结论：HMGA2在睾丸肿瘤组织中上调表达并和肿瘤进展相关，有望成为睾丸肿瘤治疗的分子靶点。 　　【关键词】高迁移率蛋白A2；睾丸肿瘤；肿瘤进展 　　Up-regulated expression of HMGA2 in testicular cancer tissue and its clinical significanceDONG Chaoxiong， YANG Liuping. Department of Urology Surgery， Guangzhou 8th People’s Hospital， Guangzhou 510440， Guangdong， China 　　【Abstract】Objectives： To investigate the expression and significance of HMGA2 in testicular cancer tissue. Methods： Using the fluorescent quantitative PCR （Q - PCR） and Western blot methods to detect HMGA2mRNA and protein in 17 cases of testicular tumors and 15 cases of normal testicular tissue， immunohistochemical （IHC） method to detect HMGA2 protein in 44 cases of testicular tumors and 30 cases of benign tissue by microarray. Results： HMGA2mRNA expression in cancerous tissue was significantly higher than that of benign group （9.09 ±2.95 VS 3.86± 1.66， P = 0.036）； HMGA2 protein expression in tumor tissue was significantly higher than that of benign organization （9.27±2.91 VS 4.66 ± 1.62， P = 0.037）. HMGA2 protein expression levels in testicular tumors were associated with clinical staging and the tumor size （P0.05）. Conclusion： Up-expressed of HMGA2 in testicular cancer is associated with tumor progression and size， which is expected to become the molecular targets of testicular tumor treatment. 　　【Key words】HMGA2； Testicular tumors； Tumor progression 　　【中图分类号】R679+.22；R737.21【文献标志码】A 　　睾丸肿瘤是青年男性最常见的恶性实体肿瘤，随着肿瘤综合治疗的进展，睾丸癌的治愈率也得到了很大程度的提高。但对于临床进展期及转移性肿瘤，临床治疗效果仍旧较差，寻找合适的肿瘤进展期肿瘤标记物以及如何评估患者的预后风险是临床上亟待解决的问题。 　　高迁移率蛋白A2（HMGA2）是在肿瘤中广泛表达的蛋白之一，其表达异常与人类多肿瘤的发生、发展及预后相关。目前HMGA2与睾丸肿瘤组织的相关性国内较少研究，我们在这方面做了一定研究，报道如下。 　　1材料和方法 　　1.1材料 　　收集近5年来医院就诊