麻杏石甘汤对A型流感病毒感染小鼠IL2IL4蛋白表达水平影响.docVIP

麻杏石甘汤对A型流感病毒感染小鼠IL2IL4蛋白表达水平影响 　　（湖南中医药大学，湖南 长沙 410208） 　　 　　摘 要：目的：观察麻杏石甘汤对A型流感病毒感染小鼠IL-2、IL-4蛋白表达水平的影响。方法：采用鼻腔接种法建立A型流感病毒小鼠肺适应株动物感染模型。实验设正常对照组、模型对照组、抗病毒颗粒组、利巴韦林组和麻杏石甘汤（MSXGD）临床等效剂量组、2倍临床等效剂量组。经灌胃给药或给生理盐水7天后，用ELISA法检测小鼠血清中IL-2、IL-4的含量水平，用免疫组织化学法定位与半定量检测小鼠肺组织中IL-2、IL-4的蛋白表达水平。结果：流感病毒感染小鼠后，IL-2、IL-4的血清含量水平和肺组织蛋白表达水平降低，与正常对照组比较，差异显著（P0.01）。MXSGD临床等效剂量组、2倍临床等效剂量组小鼠IL-2、IL-4的血清含量水平和肺组织蛋白表达水平升高，与模型对照组比较，差异显著（P0.01或P0.05）。结论：麻杏石甘汤通过调节一些IL-2、IL-4的蛋白表达水平,对机体抗感染免疫发挥一定调节作用。 　　关键词：麻杏石甘汤；A型流感病毒；IL-2；IL-4 　　中图分类号：R285.5文献标识码：A文章编号：1673-7717（2011）03-0475-03 　　 　　The Effects of Maxing Shigan Decoction on the Protein Levels of IL-2 and 　　IL-4 of Mice Infected by A-type Influenza Virus 　　 　　LU Fang-guo，ZHANG Bo，YAN Jie，LI Ling，ZHU Ying-wu，LI Shan，XIANG Qin，PAN Kun 　　（Hunan University of TCM, Changsha 410208,Hunan,China） 　　 　　Abstract:Objective：To observe effects of Maxing Shigan Decoction（MXSGD） on the protein levels of IL-2 and IL-4 of mice infected by A-type influenza virus in their serum and lung tissue. Methods: The mice model of pulmonary is established by nasal inoculating A-type influenza virus, Set up normal control group, model control group, Anti-virus particle group, ribavirin group, MXSGD clinical equivalent dose group, two times clinical equivalent dose group. After gavaging drug or normal saline, To detect the levels of IL-2 and IL-4 in serum and lung tissue. Result: The levels of IL-2 and IL-4 in serum and lung tissue for mice infected by A-type influenza virus reduced, compared with the normal control group, significant difference （P0.01）. The levels of IL-2 and IL-4 increased in MXSGD clinical equivalent dose group and two times clinical equivalent dose group, compared with the model control group, significant difference （P0.01, P0.05）. Conclusion: By affecting the expression of IL-2 and IL-4 in the body, MXSGD play a certain anti-infective immune regulatory role. 　　Key words:Maxing Shigan Decoction; A-type influenza virus; IL-2; IL-4 　　 　　流感病毒（infl