连接SelS基因的携P―选择素单抗靶向微泡结合超声治疗早期糖尿病肾病的实验研究.docVIP

连接SelS基因的携P―选择素单抗靶向微泡结合超声治疗早期糖尿病肾病的实验研究 　　[摘要] 目的 研究?B接SelS基因的携P-选择素单抗靶向微泡结合超声治疗早期糖尿病肾病的效果。 方法 选取SPF级60只雄性SD大鼠为研究对象，制备2型糖尿病大鼠模型后随机分为对照组、单纯SelS组（SelS组）、SMBp组、超声+SMBp组（UE+SMBp组），各15只。比较四组大鼠摄食量、摄水量、尿量、体重、空腹血糖、24 h尿蛋白、β2微球蛋白、血尿生化指标及P-选择素、AngⅡ、TGF-β1、SelS水平。 结果 四组大鼠治疗前一般情况比较，差异无统计学意义（P 0.05）；四组治疗后空腹血糖、24 h尿蛋白、β2微球蛋白水平、血尿生化指标及P-选择素、AngⅡ、TGF-β1、SelS水平差异有统计学意义（P 0.05）。 结论 连接SelS基因的携P-选择素单抗靶向微泡结合超声治疗早期糖尿病肾病效果确切，安全性高，可作为防治早期糖尿病肾病的有效手段。 　　[关键词] P-选择素；单抗靶向微泡；糖尿病肾病 　　[中图分类号] R587 [文献标识码] A [文章编号] 1673-7210（2017）11（a）-0015-05 　　Experimental study of monoclonal antibody targeted microbubble carrying P-selectin and connecting SelS gene in combination with ultrasound in treating early diabetic nephropathy 　　WANG Yan1 LI Jing2▲ HUANG Ruizhu3 HUANG Ping1 HUANG Yuyu2 　　1.Department of Geriatric Endocrinology， Guangzhou No.1 Peoples Hospital， Guangdong Province， Guangzhou 510180， China； 2.Department of Geriatric Nephrology， Guangzhou No.1 Peoples Hospital， Guangdong Province， Guangzhou 510180， China； 3.Department of Internal Medicine， 458th Hospital of Guangzhou Military Area， Guangdong Province， Guangzhou 510180， China 　　[Abstract] Objective To study the effect of monoclonal antibody targeted microbubble carrying P-selectin and connecting SelS gene in combination with ultrasound in treating early diabetic nephropathy. Methods A total of 60 specific pathogen free SD male rats were selected as the research objects to build type 2 diabetes rat models. Then the rat models were randomly divided into the control group， SelS group， SMBp group and UE+SMBp group， each group had 15 rats. The food intake， water intake， urine volume， body weight， fasting blood-glucose， 24 h urine protein， β2 microglobulin， blood and urine biochemical indexes， P-selectin， Ang Ⅱ， TGF-β1 and SelS levels of rats in the four groups were compared. Results Before treatment， the differences in general conditions of rats among the four groups were not statistically significant （P 0.05）. The differences in fastin