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COPLBI-1056; NO. OF PAGES 6
Available online at
Sequencing-based genome-wide association study in rice
Bin Han and Xuehui Huang
Rice, which has a modest genome size, is a wonderful model genetic dissections of the traits were performed mainly
crop that has been comprehensively studied in functional through classical biparental cross mapping. However, due
genomics. With vast germplasm resources and reduced to limits in both recombination numbers and traditional
sequencing cost, genome-wide association study (GWAS) has genotyping methods, quantitative trait loci (QTLs) were
been feasible to explore the allelic variation underlying generally mapped with a low resolution (typically with
agronomic traits in rice. In this review, we firstly summarize the several mega bases in distance) [3,4].
work on constructing GWAS platforms in human genetics and
inArabidopsis and maize, from which we have a lot to learn. We The advent of the next generation sequencing (NGS)
then discuss the sequencing strategies and experimental technologies allows genome-wide genetic variation dis-
designs for GWAS of agronomic traits in rice, and the covery and genotyping in a highly efficient way. NGS has
perspective and challenges coupled with GWAS work are been applied in genotyping of biparental cross mapping
further addressed. populations, which greatly reduced laborious works and
Addresses increased the resolution of QTL mapping [5,6]. To
National Center for Gene Research, Institute of Plant Physiology and explore the allelic variation in a broader scope for exten-
Ecology, Shanghai Institutes for Biological Sciences, Chinese
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