肉鸡MYCN基因在ALV-J病毒攻毒前后不同组织中.PDFVIP

实验研究 MYCN ALV-J mRNA 刘 强，张英英，范贤聪，魏曼莉，吕智超，秦 宁，景 阳，牟 芳，徐日福 130118 MYCN MYCN J HPRS-103 1 AA RT-PCR 8 MYCN mRNA MYCN mRNA 8 8 MYCN mRNA MYCN MYCN mRNA The expression analysis of MYCN gene mRNA in different tissues of broiler after ALV-J virus attacked. LIU Qiang ，XU Ri-Fu** ，ZHANG Ying-Ying ，FAN Xian-Cong ，WEI Man-Li ，LU Zhi-Chao, QIN Ning, JING Yang, MU Fang （Jilin agricultural university, college of animal science and technology ，Changchun 130118，China ） Abstract ：MYCN gene was first discovered in mammalian embryos and can regulate many oncogenic or tumor suppressor gene activity and closely related to cell proliferation and differentiation. To study the role of MYCN gene in the process of tumor diseases in poultry, the experiment with white f broiler as materials, the avian leukemia virus subgroup J (HPRS-103) inoculated at 1 day old broiler AA white feather artificially. The relative expression of MYCN gene mRNA in eight kinds of tissues before and after virus attacked was detected by semi-quantitative RT-PCR. The results showed that: MYCN gene mRNA in eight types of heart and liver tissues showed a wider distribution, but abundance exist a certain difference. Experimental group compared with the control group, the relative expression levels of MYCN gene mRNA in three time periods of 8 different tissues is higher in the experimental group, and showed a decline after rising trend. Through the above research can speculate that MYCN gene may play a promoting role of tumor cell proliferation in the No.2011AA100305No No.CARS-42-Z0