反向遗传学技术在猪瘟病毒研究中的应用.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2009, October 25; 25(10): 1441-1448 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2009 Institute of Microbiology, CAS CSM, All rights reserved 综 述 刘大飞, 孙元, 仇华吉 , 150001 : 猪瘟目前在许多国家流行并对养猪业造成巨大损失。虽然常规疫苗(如中国猪瘟兔化弱毒疫苗, 即C 株)在猪瘟 防控中发挥巨大作用, 但近年来在猪瘟防控中出现的新情况, 如非典型感染、持续性感染及免疫失败等; 同时目前世界 上许多国家正开展的猪瘟扑灭计划使得弱毒疫苗的应用受到很大限制。因此, 加强猪瘟病毒在致病机理、传播机制等方 面的研究以及加快新型猪瘟疫苗的开发是当务之急。近年来, 反向遗传学技术的发展为猪瘟病毒基因功能研究和疫苗制 备方面开辟了新思路。以下回顾了反向遗传操作技术在猪瘟病毒基因功能研究与标记疫苗株构建方面的研究进展, 同时 提出了该领域目前面临的问题, 并对其未来发展方向进行了展望。 : 猪瘟病毒, 反向遗传学技术, 功能研究, 标记疫苗 Applications of reverse genetics in studying classical swine fever virus Dafei Liu, Yuan Sun, and Huaji Qiu Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150001, China Abstract: Classical swine fever (CSF), caused by classical swine fever virus (CSFV), has been epidemic or endemic in many countries, and causes great economical losses to pig industry worldwide. Attenuated vaccines (such as C-strain) have played an important role in the control of CSF. Recently some new phenomena appear, such as atypical and persistent infections of CSF, immunization failure and