土壤中正己醇降解菌的多样性研究-食品科学.PDF

※生物工程 食品科学 2010, Vol. 31, No. 09 177 张锦华，师俊玲* (西北农林科技大学食品科学与工程学院，陕西杨凌 712100) ：目的：筛选能够有效降低正己醇含量的微生物菌种，了解自然界中正己醇降解菌的分布与多样性。方 法：以正己醇生产厂废水排放口周围的土壤为分离源，通过富集培养和分离筛选。结果：获得了6 株对正己醇有 显著降解作用的菌种，根据其在PDA 培养基上的菌落形态和孢子形态初步分类鉴定为镰孢菌属、地霉属、毛霉属 和曲霉属。其中，地霉属的菌株最多(3 株) ，对正己醇的降解能力强，适用pH 值范围广。地霉属菌株S12 和S13 在中性和酸性条件下对3g/L 正己醇的降解率均达60% 以上。结论：自然界中的正己醇降解菌在种类、生态、降 解能力、作用条件等方面都具有丰富的多样性。 ：正己醇；多样性；正己醇降解菌；分离；筛选；分类鉴定 Diversity of Hexanol-degrading Strains in Soil ZHANG Jin-hua ，SHI Jun-ling* (College of Food Science and Engineering, Northwest AF University, Yangling 712100, China) Abstract ：Hexanol is an unfavorable flavor in food. Too much hexanol in food is harmful for human health. Screening and understanding diversity of hexanol-degrading microorganisms are helpful for biological degradation of hexanol and its potential application in food industry, which will provide basic information for further research on hexanol-degrading mechanisms. Soil around wastewater outfalls of hexanol-producing plants was used as the resources to screen hexanol- degrading microorganisms. After enrichment and isolation, 6 strains with degradation capability for hexanol were obtained. Based on morphological characteristics of colonies and spores, 6 strains were classified as fungi, which belonged to Fusarium Link, Geotrichum Link, Mucor Link and Aspergillus P. Micheliex Link, respectively. Geotrichum Link had 3 isolated strains, which exhibited higher hexanol-degrading capacity and wider suitability of pH. The strains in Geotrichum Link, S12 and S13 could result in the degradation of 3 g/L hexanol up to 60% under both neutral and acidic conditions. The rich diversity of hexanol-degrading strains