扩展青霉脂肪酶的生产及其在外消旋烯丙醇酮拆分-高校化学工程学报.PDF

22 1 No.1 Vol.22 2008 2 Journal of Chemical Engineering of Chinese Universities Feb. 2008 1003-9015(2008)01-0166-06 , ( , 310027) (4--3- -2-(2-)-2--1- allethrolone)Rodamine B Penicillium expansum PED-03 0.5%4% C/N 0.5 Tween-80 0.05% Tween-80 55% 500 r⋅min− 1 P. expansum PED-03 36 h (C)96%(eeP)99% Q556 Q814.9 A Production of the Lipase from Penicillium expansum PED-03 and its Application in the Resolution of Racemic Allethrolone DAI Da-zhang, XIA Li-ming (Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China) Abstract: The resolution of racemic allethrolone (4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-one) catalyzed by lipase instead of chemical methods was investigated. A high-yield lipase producing strain Penicillium expansum PED-03 was obtained by Rhodamine B plate screen. It is suitable for the lipase production with 0.5% starch as carbon source and 4.0% soybean meal as nitrogen source in submerged fermentation. Tween-80 is an effective enhancer for lipase production, and the lipase activity can be increased 55% when 0.05% Tween-80 was added to the medium. Agitation speed plays an important role in the lipase production, and the suitable agitation speed is 500 r⋅min− 1. The resolution of racemic allethrolone catalyzed by the lipase from P. expansum PED-03 was carried out in a nonaqueous medium, and after reacting for 36 hours, the conversion reaches 96% of its theoretical value and the enantiomeric excess of product (eeP) reaches 99%. It shows that the enzymatic resolution method proposed presents a good