重组人类生长激素改善蛋白质营养不良机制的研究-首都医科大学.PDFVIP

2005 8 Aug. 2005 26 4 Journal of Capital University of Medical Sciences Vol. 26 No. 4 重组人类生长激素改善蛋白质营养不良机制的研究 文煜冰 刘惠兰 黄丽荣 姚 英 ( ) : ( rhGH) ,rhGH, (Alb) (Trf) (IGF ) IGF 1(IGFBP1) IGF 3(IGFBP3) 1) rhGH AlbT rfIGF ,2 ( P 0.01) 2) rhGH IGFBP1 , IGFBP3 ,2 ( P 0.05) rhGH AlbTrfIGFI ( ) , IGFBP1 , IGFBP3 : ; ; : R 459. 1 Mechanisms of Recombinant Human Growth Hormone Improving Protein Malnutrition: a Study in itro Wen ubing, Liu Huilan, Huang Lirong, ao ing ( Dep ar tment of N ep hr ology , Fux ing H osp ital , Cap ital University of M edical Sciences ABSTRACT: Objective To investigate the effects of rhGH on biosynthesis and secretion of secretory albumin, transferrin, insulinlike growth factor (IGF ) , insulinlike growth factor binding protein1, 3(IGFBP1, IGFBP3) of primary cultured hepatocytes and liver nonparenchymal cells of rat. Methods Rat hepatocytes and liver nonparenchymal cells are isolated by two step procedures in situ. RhGH is added to the medium and the vehicle is added for control. Albumin and transferrin of medium are measured with sandwich ELISA. IGF , IGFBP1, and IGFBP3 in cells lysate are analyzed by Western Blotting. Results The medium concentrationsof albumin and transferrin and the IGF level in cells lysate are significantly higher in rhGH group than in control group, all P 0. 01. The IGFBP1level in cells lysate is significantly lower and IGFBP3 level is significantly higher in rhGH group than in control group, both P 0. 05. Conclusion rhGH increases biosynthesis and/ or secretion of secretory albumin, transferrin, and IGFI et al. And rhGH inhibits biosynthesis of IGFBP1 in hepatocytes and enhances expression of IGFBP3 in nonparenchymal liver cell significantly. Key words: recombinant human growth hormone; protein malnutrition; hepatocyte 58%()