转BAR基因水稻的抗性遗传生理特性和农艺性状研究.PDFVIP

浙江大李李报(农业与生命科学版) 32(4):355^-359,2006 JournalofZhejiangUniversity(Agric.LifeSci.) 文章编号:1008-9209(2006)04-0355-05 转BAR基因水稻的抗性遗传、生理特性和农艺性状研究 段发平，郑枫，段 俊，黎垣庆，梁承邺 (中国科学院华南植物园，广州 510650) 摘 要:为了明确BAR基因对水稻的影响，对转BAR基因的BengalHU-10,BengalHC-11,Cypress PB-6,CypressPB-13的抗性遗传、生理特性和农艺性状进行了研究，结果表明，它们对除草剂Liberty的 抗性均受一对显性核基因控制，在这4个实验材料中，BAR基因在染色体上的整合位置互不等位，株 高、生育期和每穗粒数等农艺性状也不相同.转BAR基因水稻与原亲本比较，叶片中叶绿素含量、光合 速率和叶绿素荧光参数、GS酶活性等均未有明显差异，但用Liberty处理2d后，上述生理性状变化明 显，且在这4个转BA尺基因材料之间也有显著差异，说明与BAR基因的插入位置有关， 关 键 词:转BAR基因水稻;抗性遗传;生理特性;农艺性状 中图分类号:Q81 文献标识码 :A DUANFa-ping,ZHENGFeng,DUANJun,LIYuan-ging,LIANGCheng-ye(SouthChinaBotanic Garden,ChineseAcademyofSciences,Guangzhou510650,China) Resistantinheritance,physiologicalandagriculturalcharactersofBAR-transgenicrice.JournalofZhejiang University(Agric.邑LifeSci.)，2006，32(4):355-359 Abstract:InordertoinvestigatetheeffectofBARgeneonrice,thecharacteristicsofinheritance,physiology andagricultureof4BAR-transgenicrice(BengalHU-10,BengalHC-11，CypressPB-6,CypressPB-13)were studied.Theresultsindicatedthattheresistanceofthis4BAR-transgenicricetoherbicide(Liberty)-was controlledbyasingledominantnucleargene.TheinsertedlocusofBARgeneandagriculturalproperties includingplantheight,growthperiodandgrainnumberperpanicleweredifferentamongthe4BAR-transgenic rice.Therewerenoevidentdifferencesinchlorophyllcontent,photosyntheticrate,parametersofchlorophyll fluorescenceandglutaminesynthetaseactivityetcinleavesbetweenBAR-transgenicriceandtheoriginal parents.However,theabovephysiologicalcharacterschangedsignificantlyandweresignificantlydifferent betweeneachBAR-transgenicriceinleaves2dayslateraftersprayingLiberty.Theaboveresultsshowedthat thechangeofphysiologicalcharacterswasrelatedtotheinsertedlocusofBARgene. Keywords:BAR-transgenicrice;resistantinheritance;physiolog