包头泉山金矿浸矿酸性微生物群落优势度变化的比较研究-微生物学报.PDF

微生物学通报 Jan. 20, 2014, 41(1): 43−49 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.130087 研究报告 包头泉山金矿浸矿酸性微生物群落优势度变化的比较研究 聂立 税刘杨 王建英* ( 014010) 摘 要：【目的】研究不同矿石组成对微生物群落结构的影响。【方法】选取包头泉山金矿的酸 性矿坑水(Acid mine drainage ，AMD)对4 种不同组成的矿石样品进行浸矿，采用 16S rRNA-PCR 和RFLP 相结合的方法，研究浸矿前后浸矿微生物种群优势度的变化情况。【结果】所选取的3 个酸性矿坑水考查点之间浸矿微生物的种类及数量相似度较大，浸矿微生物结构相对变化度不 大。H71 和F11 相对其他两个样品的群落结构差异较大，而J72 和S71 几乎一样，说明在钾长 型和石英型矿石的选择压力下，浸矿微生物的被选择趋势是大致相同的。【结论】浸矿前后样 品中浸矿微生物的系统发育分析结果表明，所考查的克隆子的序列可分为两大分支： Acidithiobacillus 菌属和一个相对独立的菌属，且这两个分支内部菌株之间的发育距离较近。 关键词：AMD ，优势群落，浸矿微生物，重金属浸出 Comparison of bacterial communities in the Baotou Quanshan gold before and after acid leaching * NIE Li SHUI Liu-Yang WANG Jian-Ying (School of Mathematics, Physics and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou , Inner Mongolia 014010, China) Abstract: [Objective] Influence on bacterial communities of different ore composition. [Methods] The change of acidic microbial community dominance before and after leaching ore were investi- gated by using 16S rRNA-RFLP methods. [Results] AMD of three sampling points are the inde- pendent Acidithiobacillus bacteria genera of microbes, which accounts for about half the number of tested strains, three sample points between ore leaching microbial species and quantity larger simi- larity. Meantime, we