两种血小板制品细菌培养的的结果分析.docVIP

两种血小板制品细菌培养的的结果分析 　　[摘要] 目的 探讨单采血小板与混合血小板在血液采集制备过程中细菌污染的危险程度，为血小板的采集制备过程中改良工艺，提高输血安全性提供可靠数据。 方法 采用生物梅里埃公司生产的BaeT/Alert 3D 全自动细菌培养仪及应用需氧瓶（BPA Culture Bottle）、厌氧瓶（BPN Culture Bottle）对5210例单采血小板，1653例混合血小板进行了细菌培养，细菌培养阳性者转种，转种阳性者进行细菌鉴定。 结果 在5210例单采血小板细菌培养经确定阳性6例，阳性率为1.15‰；1653例混合血小板中细菌培养经确定阳性10例，阳性率6.05‰。 结论 混合血小板细菌培养阳性率（6.05‰）高于单采血小板（1.15‰），两者比较差异有统计学意义（P＜0.01）；血小板细菌污染情况仍然严峻，应引起注意。 　　[关键词] 单采血小板；浓缩血小板；混合血小板；细菌污染；细菌培养 　　[中图分类号] R446.5#8195;#8195;#8195;[文献标识码] B#8195;#8195;#8195;[文章编号] 2095-0616（2014）11-119-03 　　 　　Analysis of bacterial culture results of two types of platelet products 　　LIN#8195;Dong1#8195;#8195;CUI#8195;Siping1#8195;#8195;SU#8195;Wanlan1#8195;#8195;CHEN#8195;Baochan2#8195;#8195;YE#8195;Zhujiang1 　　1.Dongguan Central Blood Station,Dongguan 523930,China;2.Department of Blood Transfusion,Dongguan Donghua Hospital, Dongguan 523110,China 　　[Abstract] Objective To investigate the criticality of bacterial pollution in the blood collection and preparation process of single donor platelets and mixed platelets in order to provide reliable data for improving the technology in the collection and preparation process of platelets and improving the blood transfusion safety. Methods The BaeT/Alert 3D Full-Automatic Bacterial Culture System produced by BioMérieux and the BPA Culture Bottle and BPN Culture Bottle were used for the bacterial culture of 5210 cases of single donor platelets and 1653 cases of mixed platelets.The samples showing g positive bacterial culture results received subcultivation and the subcultivated sample showing positive results received bacterial identification. Results Of the 5210 cases of single donor platelets,bacterial culture confirmed 6 positive cases,with the positive rate of 1.15‰.Of the 1653 cases of mixed platelets,bacterial culture confirmed 10 positive cases,with the positive rate of 6.05‰. Conclusion The positive bacterial culture rate of mixed platelets (6.05‰)is higher than that of the single donor platelets (1.15‰), wit