后十字韧带重建后移植物组织学与胶原表型的变化.docVIP

后十字韧带重建后移植物组织学与胶原表型的变化 2006年1月第26卷第1期ChinJOrthop』塑班!12,!j 后十字韧带重建后移植物组织学 与胶原表型的变化 王健敖英芳 ? 47? ? 基础研究? 【摘要】目的探讨采用半腱肌腱重建后十字韧带(posteriorcruciateligament,PCL)后移植物的组 织学与胶原表型的变化.方法取2O只成年新西兰大白兔.实验组l8只切除右膝PCL后即刻用双股 自体半腱肌腱重建.实验组于术后3,6,52周各处死动物2只,l2,26周各处死动物6只,切取半腱肌腱 移植物;对照组2只直接切取半腱肌腱和PCL.标本采用HE,甲苯胺蓝以及I,Ⅲ型胶原免疫组化染色 观察移植物的组织学与胶原表型变化.并与正常半腱肌腱和PCL进行比较.结果常规组织化学染色 显示正常半腱肌腱和PCL在细胞构成上有明显的差别,PCL内可见软骨样细胞;重建后的移植物经过 坏死,细胞重新长入,胶原形成和重塑阶段,52周时形成的结构类似于正常PCL.但在纤维排列上与正 常PCL仍有差异.免疫组织化学染色结果显示正常PCL纤维内部仅有I型胶原表达.重建韧带I型胶 原染色随时间延长从少到多,Ⅲ型胶原染色从多到少,52周时移植物内局部仍有表达.结论采用半腱 肌腱重建PCL52周时移植物和正常PCL结构相似,但仍有差异. 【关键词】膝关节;后交叉韧带;移植物;胶原;兔 1hehistologicalandcollagenphenotypechangesofasemitendinosusautograftafterposteriorcruci- ateligamentreconstructioninrabbitNG胁A0Ying-fang.Instituteof5portsMedicine.theThird HospitalofPekingUniversity,Be~iing100083,China 【Abstract】ObjectiveToinvestigatethehistologicalchangesandcollagenphenotypeofasemi. tendinosusautograftafterreconstructionofposteriorcruciateligament(PCL1inrabbits.MethodsTwenty skeletallymatureNewZealandwhiterabbitsweredividedintotwogroups.Ingroup1.thePCL0ftheright kneesofl8matureNewZealandwhiterabbitswereresectedandimmediaterlyreconstructedwithdouble— bandsemitendinosusantograft.Thentherabbitswererespectivelykilledat3,6,12.26and52weeksafter theoperation,toobtainthethesemitendinosusautografts.Group2consistingoftheother2rabbitswas served8,8contrulgroup.andsemitendinosustendonsandPCLwereobtained.AUspecimenswerestaining withhematoxylinandeosin,toluidineblueandimmunohistochemicalmethodsoftypeI.IIIcoUagenstoan- alyzethehistologicalchangesandcollagenphenotypeofasemitendinosusautograft,andcomparethosewith normalsemitendinosustendonsandPCL.ResultsHistologicalresultsdemonstratedthatthereWSSobvious differenceatthecellformationbetweennormalsemitendinosustendonandPCL.Afterpestoperative52 weeksthegraftwassimilartOthenormalPCLwhilethealignmentofgraftwasstilldifferentfromthatof normalPCLthroughnecrosis,cellgrowingin,collagenformationandremolding.Immunohistochemistryresult demonstra