东方百合‘Sorbonne’鳞片离体培养的研究.docVIP

东方百合‘Sorbonne’鳞片离体培养的研究 　　摘要 以东方百合品种‘Sorbonne’鳞片为外植体，研究不同因子对‘Sorbonne’消毒、诱导、增殖、生根等的影响。结果表明：‘Sorbonne’外层鳞片消毒最为困难，而中层和内层差异不大；最佳消毒方法是外层鳞片用75%酒精处理30 s再用0.1%升汞浸泡18～20 min；中层和内层鳞片用75%酒精处理30 s，再用0.1%升汞浸泡14～16 min，其中不同部位鳞片诱导率从高到低分别为外层中层内层，其中外层的诱导率为76.7%；百合鳞片最佳增殖培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L，增殖系数为6.8；最佳生根培养基为1/2MS+6-BA 0.5 mg/L+NAA 0.2 mg/L，平均生根数为20条。 　　关键词 Sorbonne；鳞片；离体培养；组织培养 　　中图分类号 S682.2+9 文献标识码 A 文章编号 1007-5739（2014）22-0136-02 　　Study on Bulb Scale Culture in Vitro of Lilium oriental Hybrids ′Sorbonne′ 　　HUANG Min LIANG Chun-hui YUE Hai-lin LI Rong-xi GUO Xiao-zhen WANG Li-ping LI Xiu-ping * 　　（Department of Tropical Crops，Guangdong AIB Polytechnic College，Guangzhou Guangdong 510507） 　　Abstract The bulb scales of Lilium oriental Hybrids ′Sorbonne′ were used as explants for in vitro culture. The results showed that the best disinfection method of the outer bulb scale explants of ′Sorbonne′ was 75% ethanol for 30 s，followed by 0.1% HgCl2 18～20 min，and the best disinfection method of the middle scale and the inner scale bulb scale explants of ′Sorbonne′ was 75% ethanol for 30 s，followed by 0.1% HgCl2 14～16 min. The most difficult was the outer scale disinfection，but difference of disinfection effect of the middle scale and the inner scale was smaller. The scales in different parts had different rate of induction of bulbs as follows：the outer scalesthe middle scalesthe inner scales. The induction rate of the outer scales was 76.7%. The appropriate medium for multiplication was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L with the multiplication coefficient of 6.8；the best medium for root induction was 1/2MS+6-BA 0.5 mg/L+NAA 0.2 mg/L and the number of roots per plantlets of 20. 　　Key words Sorbonne；bulb scale；culture in vitro；tissue culture 　　百合（Lilium spp.）是多年生草本球根植物，品种繁多，栽培历史悠久，是世界名贵花种之一。利用天香百合（L. auratum）的花型和药百合（L. speciosum）的艳丽花色及其芳香宜人的遗传特性，培育出的东方百合杂种（L.tenuifolium oriental hybrid）群，花型靓丽，色泽鲜艳，是目前栽培的主要新品种，在切花生产中占据重要地位[1]。东方百合是通过杂交育种选育出来的，因此种子高度败育，常采用传统的鳞茎球繁殖方法，繁殖速度缓慢，在短