HPLC法测定金银花中8种成分量.PDFVIP

HPLC 法测定金银花中 8 种成分的量 1, 2 1, 2 1, 2 1, 2 1, 2 1, 2 1, 2* 李 淼 ，王永香 ，孟 谨 ，付小环 ，毕宇安 ，王振中 ，萧 伟 1. 江苏康缘药业股份有限公司，江苏 连云港 222001 2. 中药制药过程新技术国家重点实验室，江苏 连云港 222001 摘 要：目的 建立测定金银花药材中新绿原酸、绿原酸、隐绿原酸、咖啡酸、异绿原酸A 、异绿原酸B、异绿原酸C、断氧 化马钱子苷8 种成分的HPLC 方法。方法 采用RP-HPLC 法，色谱柱为Luna 5 μ C18 柱（250 mm ×4.6 mm，5 μm ）；流动相为 甲醇-0.1%磷酸水溶液，梯度洗脱，0～20 min，12%～30% A；20～60 min，30%～50% A，体积流量1.0 mL/min；检测波长237、 324 nm，柱温30 ℃。结果 8 种成分均达到基线分离，各成分均有较宽的线性范围和良好的线性关系（r ＞0.999 9 ）；回收率 在98.72%～102.50%。结论 本方法准确灵敏、重复性好，能较全面地评价金银花药材的质量。 关键词：金银花；新绿原酸；绿原酸；隐绿原酸；咖啡酸；断氧化马钱子苷 中图分类号：R286.022 文献标志码：A 文章编号：0253 - 2670(2014)07 - 0 - 05 DOI: 10.7501/j.issn.0253-2670.2014.07. Determination of eight components in Lonicera japonica by HPLC LI Miao1, 2, WANG Yong-xiang1, 2, MENG Jin1, 2, FU Xiao-huan1, 2, BI Yu-an1, 2, WANG Zhen-zhong1, 2, XIAO Wei1, 2 1. Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China 2. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China Abstract: Objective To establish an HPLC method for simultaneously determining eight components such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, and secoxyloganin in Lonicera japonica . Methods An RP-HPLC method was established with Luna C18 (250 mm × 4.6 mm, 5 μm) column by a gradient elution using methanol and 0.1% phosphoric acid; The flow rate was 1.0 mL/min with double wavelength (237 and 324 nm), and the column temperature was 30 ℃. Results Baseline of eight components was separated; Each component had a wide linear range and a good linear relationship (r 0.999 8); the recoveries were 98.72% to 102.50%. Conclusion The method is accurate, sensitive, reliable, and with good reproducibility. It could be used for the quality control of L. ja