尿中多胺的毛细管电泳激光诱导荧光测定法-中南大学学报医学版.PDFVIP

BU LL HUNAN MED UNIV 2003, 28( 5) 495 1 1 2 1 1 荆照政 , 荆施展 , 陈 松 ,李建玲 ,肖志强 ( 1. 中南大学湘雅医院中心实验室, 长沙410008; 2. 湖南省科技厅社会发展处 , 长沙410001) [] 目的:建立高效毛细管区带电泳( CZE) 激光诱导荧光( LIF) 测 人尿中多胺(POL) 的新方法方法: 样品用5 mmol/ L 的HClO 分离蛋白, 样品中多胺与异硫氰酸荧光素( FITC) 完成衍生反应后, 用四甲基乙二胺 4 ( TEMED) 作内标,在 15 mmol/ L 的硼酸盐缓冲液(pH 8.5) 中, 运用毛细管区带电泳进行色谱分离, 以激光诱导荧 光测 法测 多胺结果: 多胺测 在 10 200 g/ L 范围内具有良好的线性关系( r = 0.996) , 最低检测限为 16 g/ L , 日内变异系数(CV) 3.51% 4. 61% , 日间变异系数( CV) 3.74% 4. 83% , 方法平均回收率为96. 00% 99. 33% 结论: 本方法简便快速 量可靠, 可用于临床实验室测 人尿中多胺水平 [] 多胺; 毛细管区带电泳; 激光诱导荧光测 ; 方法 [ ] R965. 2 [ ] A [ ] 2003) Determination of polyamines in urine by capillary electrophoresis with laser induced f luorescence 1 1 2 JING Zhaozheng , JING Shizhan , CHEN Song , et al . ( 1. Ce ntral L aboratory, X iangy a H osp ital, Cent ral S outh Univ ersity , Changsha 410008, China 2. Dep art men t of Social Dev elop ment , H u na n Polytech nical Of f ice, Changsha 410001, China Abstract: Objective To establish a new method to determine polyamines (POL) in urine using high performance capillary zone electrophoresis ( CZE) with laser induced fluorescence. Methods Pro tein in the urine sample was separated by 5 mmol/ L HClO4, and then fluoresisothiocyanate was added for the derivative reaction with POL in the sample. The N, N, N NT etramethylethylenediamine was added to the sample as the internal standard. T he POL was separated by CZE and laser induced fl