子宫内膜息肉患者息肉组织中ERPRVEGFMVD表达的研究.docVIP

子宫内膜息肉患者息肉组织中ERPRVEGFMVD表达的研究 　　【摘要】 目的 探讨雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)、血管内皮生成因子(vascular endothelial growth factor,VEGF)、微血管密度(microvessel density,MVD)在子宫内膜息肉组织与正常子宫内膜中的表达及意义。方法 宫腔镜下观察并行子宫内膜息肉切除的患者40例，同时取同一患者非息肉部位内膜组织，石蜡包埋制成切片，HE染色按内膜的病理类型分为良性息肉增生组和增生期子宫内膜两组，符合条件的病例28例。用免疫组织化学方法测定ER、PR、VEGF、MVD含量，采用LeicaQ500IW图像分析仪进行测定，应用光密度值进行比较。结果 息肉增生组ER、PR、VEGF、MVD含量高于增生期子宫内膜。结论 患者子宫内膜息肉增生组和增生期子宫内膜的ER、PR、VEGF、MVD含量不同，VEGF在子宫内膜息肉的发生发展中占有重要地位。? 　　【关键词】子宫内膜息肉；增生期子宫内膜；血管内皮生成因子；微血管密度 　　 　　The stundy of expression of ER、PR、VEGF and MVD in endometrial polyps and prolifefrative phase endometrium for patients with abnormal uterine blooding 　　 　　LI Jing-hua,WANG Xiao-guang,FENG Li-min,et al.Department of Obstetrics and Gynecology Tiantan Hospital,Beijing 100050,China 　　? 　　【Abstract】 Objective To evaluate the the expression of estrogen receptors(ER),progesterone receptors(PR)and vascular endothelial growth factor(VEGF)and microvessel density(MVD)in patients withendometrial polyps.Methods 28 cases of endometrial polyps included in the biopsys were divided into 2 groups by HE staining pathologic result including endometrial polyps and proliferative phase endometrium.ER、PR、VEGF、MVD were measured in immunohistochemistry.ER、PR、VEGF index were determined with a computerized image analyzer.Results Expression of ER、PR、VEGF、MVD in the group of endometrial polyps is the higher than the group of proliferative phase endometrium.Conclusion The expression of ER、PR、VEGF、MVD in glandular epithelium were significant difference in two groups(P0．01).ER,VEGF paly main function in endometrial polyps of patients with endometrial polyps.? 　　【Key words】Endometrial polyps;Proliferative phase endometrium;VEGF；MVD 　　? 　　子宫内膜息肉常常引起不规则阴道出血，前者是子宫内膜受雌激素持续作用发生局灶性增生的良性病变，若同时应用孕激素这种改变仍然不能逆转[1]；VEGF主要生理功能是促进内皮细胞分化、增生、迁移、浸润调控血管发生和维持血管内皮细胞的功能，在组织正常增生和异常增殖的发生和发展过程中起着非常重要的作用。为此，测定了上述几种因子在子宫内膜息肉及正常子宫内膜组织中的表达，探讨与子宫内膜息肉发生的关系。? 　　1 材料与方法? 　　1．1 标本选择 宫腔镜下观察并切除子宫内膜息肉患者40例，同时取同一患者非息肉部位内膜组织