硕士论文-厌氧发酵法生物制氢菌种的选育及发酵工艺的初步研究.doc.docVIP

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硕士论文-厌氧发酵法生物制氢菌种的选育及发酵工艺的初步研究.doc.doc

硕士论文-厌氧发酵法生物制氢菌种的选育及发酵工艺的初步研究.doc

⑧ 天洋大薯 中国近代第--N太学 硕士学位论文 _k'AIID,,'lm●■■0-爿昌0一■啊■■¨0■■_ 学科专业:生物化工 作者姓名:陈宇 指导教师:闻建平教授 千津士堂研窨牛畴 2005年8月 ABSTRACT Asanideal,cleanandsustainableenergysource,hydrogenhasabrightfuturein the21stcentury.Ithasmanyotherusesaswellasenergy,suchasareactantin hydrogenationprocess,azl02scavenger,afuelinrocketenginesandacoolantin electricalgeneratorstotakeadvantageofitsuniquephysicalproperties.Biological productionofhydrogen,usingorganisms,isanexcitingnewareaoftechnology developmentthatoffersthepotentialproductionofusablehydrogenfromavarietyof renewableresources. Twostrainswiththehighabilityt0producehydrogen,FIB一5,bacteria,and HY一35,yeast,wereisolatedfromtheactivatedsludge.Thenthetwostrainswere identifiedasEnterobacteraerogenesandCandidamaltosarespectivelyafterbeing investigatedthemorphologicandtaxonomicproperties.Furtherstudyshowedthat undertheoptimumfermentationconditions,thehydrogenproducingabilityofE aerogenesHB一5improvedby19.9%(Qm“)and15.8%cY),respectively. He-NelaserwasemployedtoirradiatethecellsofthestrainEaerogenesHB-5 toobtainthemutantwiththehigherhydrogenproducingability.Asteady—going positivemutantstrain,EaerogenesHB一5M,丽山Qm.x=242.0mlH2/(Lmedia‘h),Y 一1483mlH2/(Lmedia、whichwere32.2%and23.3%higherthanthoseofthewild strain,wasacquired.Moreover,thedynamicsstudyonthecellgrowth,production andsubstramdegradationindicatedthatthesimulatedvaluesagreedwellwiththe exp舐mentaldataundertheselectedmodel. Furthermore,hydrogenproductionbythemixedcultureofE.aerogenesFIB一5M andCmaltosaHY-35andtheiroptimizationofthemixedculturefermentative conditionwerealsoinvestigated.Theresultsshowedthatthemixedculturehasa higherability. Keywords:Fermentativehydrogenproduction;Enterobacteraerogenes;Candida maltosa;Lasermutation;Optimizationoffermentationcondition; synergisticeffect 独创性声蹯 李太声麓爱呈交瓣擎锭论文愚零太凌辱萝萃撂夥下避鳕豹礤究工终移取褥蕊 研究成黎,除了文中特别加以标注和致谢之处外,论文中不包禽其{旗人已缎麓 褒箴撰麓进戆磅究藏慕,也不餐含爻获褥鑫鎏态鐾羧荬镪教簿机稳熬学蹙簸 谣书焉镄周过的榜黼。与我一圊Z佟的丽惠对零研究繇做的佼俺贡献均已赢论 文中佟了疆确熬说臻舞表承了嚣意。 学谯论文俘卷签名:俯签字基鬻:翻炉工霉箩囊鸪酲 学位论文版掇使用授权书 本学链论文作者究垒了解羲连基囊有荧傺戳、使用学位论文的规窥。

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