冬前低温胁迫下白菜型冬油菜抗寒性的形态及生理特征-中国农业科学.PDF

中国农业科学 2013,46(22):4679-4687 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.22.005 冬前低温胁迫下白菜型冬油菜抗寒性的形态及生理特征 刘自刚，孙万仓，杨宁宁，王 月，何 丽，赵彩霞，史鹏飞，杨 刚，李学才，武军艳，方 彦，曾秀存 （甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室，兰州730070 ） 摘要：【目的】为白菜型冬油菜抗寒种质创新及抗寒品种选育提供依据。【方法】以抗寒性不同的白菜型 冬油菜品种为材料，采用田间观察、比色法、冰晶消长法、显微测微、光合测定等技术，探讨北方寒旱区主 要冬油菜品种抗寒性差异形成的形态、生理及分子机理。【结果】冬前低温阶段，弱抗寒品种多数气孔关闭或 半开放，而强抗寒品种（陇油6 号）气孔仍保持完全开放状态，且光合速率、根冠比、根部干物质积累均较 高；可溶性蛋白与抗寒性显著正相关，而 SOD、POD、CAT 活性、游离脯氨酸、MDA 含量与抗寒性无明显相关 关系；与天油2 号相比，低温下陇油7 号质外体蛋白提取液形成冰晶体积小、分布均匀，形态以五边形、椭 圆形和双菱形为主，具有明显重结晶抑制活性和饰晶活性。【结论】冬前低温阶段，白菜型冬油菜强抗寒品种 叶片气孔多数仍保持开放，Pn 较高，光合产物优先分配至地下部贮存；根部抗冻蛋白表达上调，增强了其抗 寒性。 关键词：白菜型冬油菜；抗寒性；形态生理特征；光合特性；抗冻蛋白 Morphology and Physiological Characteristics of Cultivars with Different Levels of Cold-Resistance in Winter Rapeseed (Brassica campestris L.) During Cold Acclimation LIU Zi-gang, SUN Wan-cang, YANG Ning-ning, WANG Yue, HE Li, ZHAO Cai-xia, SHI Peng-fei, YANG Gang, LI Xue-cai, WU Jun-yan, FANG Yan, ZENG Xiu-cun (College of Agronomy, Gansu Agricultural University/ Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou 730070 ) Abstract: 【Objective 】The objective of this study was to provide an evaluation method for the development of cold tolerant Brassica campestris germplasm. 【Method 】In this study, the plant morphological characteristics and physiological parameters were measured, and the molecular mechanism of cold-resistance of several cultivars was discussed. 【Result 】Under low temperature, the majority of the stomata in cold sensitive cultivars were closed or semi-closed. But the stomata in strong cold-tolerant cultivars (Longyou 6) maintained opening. The pho