产琥珀酸重组解脂酵母发酵副产物乙酸的代谢控制-微生物学报.PDF

生 物 工 程 学 报 高翠娟 等/产琥珀酸重组解脂酵母发酵副产物乙酸的代谢控制 389 Chinese Journal of Biotechnology /cjbcn Mar. 25, 2018, 34(3): 389−395 DOI: 10.13345/j.cjb.170299 ©2018 Chin J Biotech, All rights reserved ·工业生物技术· 高翠娟，郑亚琴 临沂大学 生命科学学院，山东 临沂 276000 高翠娟, 郑亚琴. 产琥珀酸重组解脂酵母发酵副产物乙酸的代谢控制. 生物工程学报, 2018, 34(3): 389–395. Gao CJ, Zheng YQ. Control of acetic acid metabolism of recombinant Yarrowia lipolytica for efficient succinic acid production. Chin J Biotech, 2018, 34(3): 389–395. 摘 要: 琥珀酸是一种高附加值的有机酸，广泛用于食品、化工和农药领域。解脂酵母 Yarrowia lipolytica 作为 新型强健的非传统酵母，近年来逐渐吸引了研究者的注意。前期通过基因敲除琥珀酸脱氢酶基因构建了一株产琥 珀酸的重组解脂酵母PGC01003 。由于糖酵解和TCA 循环流量不协调，PGC01003 分泌大量副产物乙酸，限制了 琥珀酸产量的进一步提高。为降低乙酸的溢出，实现自然低pH 值发酵生产琥珀酸，首先干扰旁路代谢，异源表 达来自鼠沙门氏菌的乙酰辅酶A 合酶，乙酸的产量下降至4.6 g/L ，比对照降低了24.6% 。而基因敲除乙酰辅酶A 水解酶基因得到的重组菌PGC11505 ，发酵96 h 乙酸分泌量只有0.4 g/L ，琥珀酸产量提高到7.0 g/L ，琥珀酸的 转化率为0.30 g/g ，为进一步构建高产琥珀酸的细胞工厂奠定基础。 : 解脂酵母，发酵，琥珀酸，乙酸 Control of acetic acid metabolism of recombinant Yarrowia lipolytica for efficient succinic acid production Cuijuan Gao, and Yaqin Zheng School of Life Sciences, Linyi University , Linyi 276000, Shandong, China Abstract: Succinic acid is a high value-added organic acid widely used in food, chemical and pesticide industries. As a new robust non-conventional yeast, Yarrowia lipolytica attracts more and more attention due to its potential for industrial applications. Previously, we obtained a succinic acid-producing strain through gene deletion of succinic acid dehydrogenase subunit encoding gene Ylsdh5, resulting in the strain of PGC01003. However, the recombinant strain produced large amount of acetic acid due to imbalance between glyco