蝴蝶兰抗冷性株系的鉴定与筛选.pdfVIP

第35卷摇 第5期 北 京 林 业 大 学 学 报 Vol.35,No.5 2013年9月 JOURNAL OF BEIJING FORESTRY UNIVERSITY Sep. ,2013 蝴蝶兰抗冷性株系的鉴定与筛选 李摇 杰摇 匡摇 萍摇 王芝娜摇 张银洁 (西南科技大学生命科学与工程学院) 摘要:为了获得抗冷性蝴蝶兰株系,测定了低温胁迫及温度回升对Phalaenopsis ‘Taida Salu爷伊P. ‘Dinghan Orange Anne爷杂交后代4个不同株系及亲本的抗氧化酶活性、渗透调节物质和膜脂过氧化作用的影响,用主成分分析对各 指标的变化率进行综合评价分析。 结果表明:抗冷性程度不同的株系超氧化物歧化酶(SOD)、过氧化物酶(POD) 和过氧化氢酶(CAT)活性所表现出来的差异,随温度降低,差值呈增大趋势;抗冷性强的株系在温度回升到正常条 件时,可溶性糖、脯氨酸和丙二醛(MDA)含量、电解质渗漏率能恢复至处理前水平,而抗冷性弱的株系难以恢复。 综合评价排序表明,P6为强抗冷株系,P4、P3为中抗冷株系,其他为弱抗冷株系。 关键词:蝴蝶兰;抗冷性;生理指标 鄄鄄 鄄鄄 鄄鄄 中图分类号:S682郾31摇 摇 文献标志码:A摇 摇 文章编号:1000 1522(2013)05 0091 06 LI Jie; KUANG Ping; WANG Zhi鄄na; ZHANG Yin鄄jie. Identifying and screening cold tolerance 鄄鄄 genetypes ofPhalaenopsis varieties. Journal of Beijing Forestry University (2013)35(5)91 96 [Ch, 16 ref.] College of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang,Sichuan,621010,P.R.China. Four different offspring varieties of Phalaenopsis ‘Taida Salu爷伊P. ‘Dinghan Orange Anne爷 and their parentals were selected to compare their tolerance to coldness. Their antioxidase activities, osmoregulation substance and membrane lipid peroxidation were detected under low temperature stress and recovery. And their tolerance to coldness was evaluated comprehensively using principal component analysis. The results showed that the differences of SOD,POD and CAT activities among varieties were significant. The contents of soluble sugar,free proline and MAD,electrolytic leakage in stronger cold