蚕丝及其织物的微生物染色研究-纺织化学与染整工程专业论文.docxVIP

PAGE PAGE IV III III 中文摘要 蚕丝及其织物的微生物染色研究 果表明柞蚕彩丝结晶度和取向度均比普通柞蚕丝偏高，强力比普通柞蚕丝高 5.5%， 但伸长率减少 3.5%，初始模量升高 3.6%，普通柞蚕丝比柞蚕彩丝具有更优良的柔软 性。 2、蚕丝织物的蓝黑紫色杆菌生物染色 蓝黑紫色杆菌生物染色方法研究中，首先研究了细菌形态和培养特征，绘制了细 菌生长曲线、色素浓度标准曲线。其次，对蓝黑紫色杆菌生物染色方法进行了工艺优 化，研究了染色温度、染色时间对真丝电力纺表观得色的影响以及染色时间对上染率 的影响 ， 得出的优化工艺为 ： 用少量 斜面培养基 菌种 接种至种子培养基中， 200rpm,20℃培养 3.5d，种子液调节菌浓度为 1-2×108 cfu/mL，按照 6%接种到发酵培 养基中，200rpm，20℃培养 7d，用发酵培养液直接作为染色液，按照 1:20 的浴比， 将灭菌的真丝电力纺置于染色液中，70℃染色 3.0h 将染色后的织物取出,用纯净水冲 洗 5～10 分钟,95～98℃ 烘干。最后，对最优工艺下染色的真丝电力纺进行测试， 在此工艺下染色织物的 K/S 值为 6.0875，上染率可达 89.22%，皂洗牢度褪色 4 级， 沾色 4-5 级，干湿摩擦牢度 5 级，日晒牢度为 1 级。XPS 结果显示经过蓝黑紫色杆菌 染色的家蚕丝织物不仅含有 C、O 和 N 元素，而且出现了 S、P 元素，N 元素的含量 升高明显。 关键词：蚕丝；微生物染色；红曲霉菌；蓝黑紫色杆菌；色价；孢子计数 作 者：余莹莹 指导老师：陈国强 蚕丝及其织物的微生物染色研究英文摘要 蚕丝及其织物的微生物染色研究 英文摘要 Study on microbial dyeing of silk and silk fabrics Abstract In this paper, in order to explore a new ecological dyeing method, tussah silk and silk fabrics were dyed using biological methods. Ecological dyeing using Monascus and Janthinobacterium lividum were discussed respectively. During the dyeing process, tussah silk and silk fabrics were dipped directly in the low temperature dye bath without extraction of pigment. Skipping the traditional bottlenecks, natural colored silk fabric dyeing with nonchemical dyes meets the consumption concept of return to natural and environmental protection requirements. And its low carbon characteristic meets the national development strategy of sustainable development, and would promote the printing and dyeing industry of green ecological significantly. During the ecological dyeing using Monascus, the bacterial morphology and cultural characteristics of Monascus were investigated firstly. Then the rDNA D1/ D2 and ITS gene sequences of this strain were identified using primers of a Fungi Identification PCR Kit (Code No.RR178) from TaKaRa company. The results showed that the strain shared more than 99% homology with the Monascus reference strain. The silk fabrics dyed under optimal process were tested. The resul