蚕丝蛋白水凝胶的仿生矿化研究-材料学专业论文.docxVIP

浙江理 浙江理工大学硕士学位论文 蚕丝蛋白水凝胶的仿生矿化研究 万方数据 万方数据 细胞能在仿生矿化丝素水凝胶中正常地粘附、延伸、增殖、分化，并有早期的骨界面的形 成。说明本实验制得的生物复合材料具有良好的骨细胞相容性，且其骨细胞相容性随着矿 化产物中矿物含量增加而增强，同时，可溶性功能分子的引入也增强了材料的相容性。 本实验制备的仿生矿化丝素蛋白水凝胶因其有机-无机复合结构，克服了羟基磷灰石无 机材料脆性大，丝素蛋白有机材料骨生物活性差的缺点。利用丝素蛋白水凝胶仿生矿化得 到结构可控、机械性能和生物相容性优异的丝素蛋白/磷酸钙复合材料，为利用仿生矿化的 方法制备用于骨组织工程的类骨材料提供了方法和思路。 关键词：丝素蛋白水凝胶；仿生矿化；羟基磷灰石；生物相容性；骨组织工程 V Abstract It is well known that the biominerals have been formed in the hydrogel environment. Biomineralization is a complex process, which always influenced by the organic components in the body. The insoluble organic macromolecules and the soluble acidic proteins work together to regulate the crystal nucleation and growth, to modulate the crystal size and shape, assemble the crystals into complex structures through molecular recognition in mild environments. The unusual structure endows biominerals with excellent performance far better than synthetic materials, including unique optical properties, high strength and fracture toughness. Inspired by biomineralization process, 3D silk fibroin hydrogel is designed as a model to modulate the nucleation and growth of hydroxyapatite crystals. The mineralization process of fibroin hydrogels containing different Ca ion contents and the influence of the mineralization time are investigated in phosphate solution via a simple ion diffusion method； On the other hand, both critic acid and sericin are chose as functional molecules. We examined the effect of 3D silk fibroin hydrogel in the absence/presence of functional molecules on the mineralization process. XRD, FTIR, FE-SEM, TEM, TGA and mechanical test investigated the structure and property characteristics of biomineralizated silk fibroin hydrogel. The biocompatibility of the biomineralized hydrogel was evaluated using FE-SEM, MTT, Alamar blue and ALP examined the morphology, proliferation and differentiation of osteoblast MG-63 cells in vitro. The results of the study are as follows: Ca2+ existed in the hydrogel played an important role