超声空化效应促人骨髓间充质干细胞体内归巢的实验研究-外科学(骨科)专业论文.docxVIP

万方数据 万方数据 第二部分 超声空化效应促人骨髓间充质干细胞体内归巢的实验研究 【 目的 】 研究观察超声空化效应对慢病毒介导 hCOX-2 shRNA、 hAggrecanse-1shRNA、 hIGF-1 基因转染人骨髓间充质干细胞移植的可行性及干细胞在兔关节炎模型体内归巢的 影响。 【方法】 关节炎模型造模成功的新西兰兔分为 3 组：转染基因人骨髓间充质干细胞+微泡 +超声组（实验组）、转染基因人骨髓间充质干细胞+超声组（对照组）、转染基因人骨髓间 充质干细胞+无处理组（空白组），移植细胞后检测兔后肢膝关节滑膜组织学观察及 RT-PCR 基因检测。 【结果】 超声空化效应可以增加慢病毒介导 hCOX-2 shRNA、hAggrecanse-1shRNA、hIGF-1 基因转染人骨髓间充质干细胞在兔关节炎模型进入靶器官的比例。 【结论】 为临床应用骨髓间充质干细胞治疗遗传免疫性疾病提供了有效途径及实验基础。 硕士研究生：袁钰祺（骨科学） 指导教师 ：王英振 教授 关键词：超声；微泡；空化效应；骨髓间充质干细胞；移植 A Abstract PART ONE Lentivirus-mediated cyclooxygenase 2 and aggrecanase 1 silencing and insulin-like growth factor 1 overexpression in the cultured human bone marrow mesenchymal stem cells OBJECTIVE: To observe the expression of shRNA vectors carrying cyclooxygenase 2, aggrecanase 1 and overexpression vectors carrying insulin-like growth factor 1 in bone marrow mesenchymal stem cells. METHODS: Lentiviral vectors carrying the silencing gene cyclooxygenase 2, aggrecanase 1, the over-expressing gene insulin-like growth factor 1 and binding green fluorescent protein were constructed with recombinant lentiviral technology, and then the recombinant lentiviral vectors were used to transfect passage 3 human bone marrow mesenchymal stem cells cultured in vitro (experimental group). The human bone marrow mesenchymal stem cells transfected with no target gene lentivirals served as negative control group. The human bone marrow mesenchymal stem cells transfected with no treatment served as blank group. RESULTS AND CONCLUSION: Cyclooxygenase 2 and aggrecanase 1 transfected in human bone marrow mesenchymal stem cells were significantly inhibited at gene and protein levels, while the expression of insulin-like growth factor 1 was increased significantly at gene and protein levels. We confirmed that cyclooxygenase 2 and aggrecanase 1 were successfully silenced while insulin-like growth factor 1 overexpressed by using lentiviral vectors in human bone marrow mesenchymal stem cells, which brings a new hope for the systemic ge