茶皂素经口毒性的致突变性与亚慢性实验研究-公共卫生与预防医学专业论文.docxVIP

万方数据 万方数据 剂量范围内，茶皂素各剂量组小鼠睾丸细胞染色体畸变率与对照组比较均有增 加，但无统计学差异（P＞0.05），而阳性对照组的畸变率明显增加，差异具有 统计学意义（P0.0001），表明茶皂素不能诱导雄性小鼠生殖细胞染色体畸变。 结论：1、茶皂素对 Wistar 雌、雄大鼠的亚慢性（3 个月）经口毒性实验未观察 到 有 害 作 用 的 剂 量 (NOAEL ） 为 20mg/(kg BW) ， 最 小 观 察 到 有 害 作 用 剂 量 (LOAEL）为 100mg/(kg BW)。2、茶皂素对动物体重有一定的影响，也有一定 蓄积作用，其主要毒作用部位可能是肺部。3、三项致突变试验结果均为阴性， 表明茶皂素无明显的致突变作用。 关键词：茶皂素；亚慢性毒性；致突变性；安全性评价 II Abstract Objective: Subchronic toxicity test by oral in Wistar rats and three mutagenicity tests were adopted to ascertain the subchronic toxic characteristic and the mutagenicity of TS, then further, to provide scientific basis for the safe evaluation and the toxicological data for TS. Methods: 88 Wistar rats were divided into 4 groups by weight, animals were fed with the diets containing TS (0 mg/kg, 20mg/kg, 100mg/kg, and 500mg/kg) for 90 days. During the study, Clinical signs, body weights, food consumption, organ weights, serum biochemistry, urinalysis, and histopathology were observed and recorded. Three tests were conducted to detect the mutagenicity of TS .There were mice bone marrow polychromatic erythrocytes micronucleus, Ames assay and mice testicle cell chromosome aberration test. Results: 1.During the study, no death and obvious abnormal changes were observed for the subjects. Compared with the control group, body weights of the males in case group significantly decreased in the high-dose group (P0.05) from the 9th weeks. The WBC concentration in the males significantly increased in the high-dose group (P0.05), index of brain, spleen and lung increased (P0.05). Lung index of the males in the medium group increased (P0.05).ALB concentration of the females in the high dose group was decreased, significant increased of ALT, ALP and CHE (P0.05). 2.At the dosage from 2.5 to 50 mg/ml, micronucleus rate had no significant difference in comparison with the negative and the ratio of PCE/(PCE+NCE) above 0.5,which means TS couldn’t induce micronucleus formation and inhibit bone marrow cell-cleavage. At the 5~5000μg/pl