恩度调控人脐静脉血管内皮细胞microRNAs表达及意义-老年医学专业论文.docxVIP

The significance of microRNAs expression in HUVECs modulated by Endostar Postgraduate：Rong-ge Zhang Supervisor： Prof. Tie-jun Yin Department of Comprehensive Medicine，Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Abstract Objective To investigate the migration effects of human umbilical vascular endothelial cells（HUVECs）in vitro interfered by Endostar in different concentrations and the expressions of miR-126 、 miR-221 、 miR-222 of HUVECs, and to explore the anti-angiogenesis mechanism of Endostar by microRNAs. Methods The effect on the migration of HUVECs induced by vascular endothelial growth factor (VEGF), which were interfered with different concentrations of Endostar(50 、100 、200 、500ng/ml) , were determined by cell scratch healing assay. The expressions of miR-126 、miR-221and miR-222, which were interfered with different concentrations of Endostar(25、50 、100 、200 、500ng/ml) on different time(1/2、1、2、 4、8h), were determined by real-time fluorescent quantitation RT-PCR. To identify the effect on microRNAs in HUVECs modulated by Endostar, relative gene expression data were analyzed by 2- △△CT method. Results ① Endostar of different concentrations can inhibit the migration of HUVECs directly and reduce effect of up-regulated migration of HUVECs induced by VEGF. It was in dose dependent manner. ② Over expressions of miR-126、miR-221 and miR-222 were detected in the HUVECs. ③ The expression of miR-126 was down-regulated by Endostar significantly（0.07 ± 0.01～0.56 ± 0.04，P﹤0.01）, as compared with that interfered by Endostar before. While the expression of miR-221 and miR-222 were obviously up-regulated by Endostar（1.45 ± 0.06～5.12 ± 0.48，1.42 ± 0.19～6.68 ± 1.51，P﹤0.01）, as compared with that interfered by Endostar before. ④ To the extent of obvervation, the expressions of miR-126、miR-221 and miR-222 was not in dose/time dependent manner by Endostar regulating. Conclusion Endostar