二氢杨梅素调控自噬保护高糖诱导血管内皮细胞损伤的作用研究-营养与食品卫生学专业论文.docxVIP

MCP-1 monocyte chemotactic protein 1 单核细胞趋化蛋白-1 MDA Malondialdehyde 丙二醛 PAGE Polyacrylamide gel electrophoresis 聚丙烯酰胺凝胶电泳 PVDF Polyvinylidene Fluoride 聚偏二氟乙烯 PMSF Phenylmethanesulfonyl fluoride 苯甲基磺酰氟 PBS Phosphate buffered saline 磷酸盐缓冲液 RAPA Rapamycin 雷帕霉素 Raptor regulatory associated protein of mTOR mTOR 调控相关蛋白 RIPA Radio Immunoprecipitation Assay 细胞组织快速裂解液 ROS Reactive oxygen stress 活性氧 SIRT1 Sirtuin type 1 去乙酰化酶 SOD Superoxide dismutase 超氧化物歧化酶 TEM Transmission electron microscopy 透射电子显微镜 TNF-α Tumor necrosis factor-α 肿瘤坏死因子 α 2 Dihydromyricetin regulates autophagy to protect human umbilical vein endothelial cells from high glucose-induced injury Abstract Diabetes has been considered as the third-leading health threats after cardiovascular diseases and cancer worldwide. Diabetic vascular complications including diabetic heart diseases, diabetic retinopathy etc, are the leading cause of morbidity and mortality among people suffering from diabetes, and high glucose-induced injury in vascular endothelial cells is a major initiator of diabetic vascular complications. Thus, it is necessary to further clarify the underlying mechanisms for diabetic vascular complications, and to explore the corresponding strategies for prevention and treatment of diabetic vascular complications. Autophagy as a lysosomal degradation pathway of cytosolic components such as proteins and organelles plays an important role in the maintenance of cellular homeostasis. The occurrence of autophagy in response to environmental stress by self-regulatory mechanisms is generally regarded as a cell survival mechanism. However, impaired or excessive autophagy induced by certain stress factors may lead to cell death. Autophagy is considered as a double-edged sword in cell survival and death. Therefore, it is very important to elucidate the role of autophagy in high glucose-induced injury in vascular endothelial cells, the resolution of this problem has major implications in the design of autophagy-ta