嗜麦芽寡养单胞菌临床株外排泵SmeEF诱导表达的研究..doc

嗜麦芽寡养单胞菌临床株外排泵SmeDEF诱导表达的研究 ：孙二琳宋诗铎祁伟王玉宝 】目的了解嗜麦芽寡养单胞菌的临床株H407和 A26在抗菌药物诱导下耐药性和SmeDEF外排泵表迗的变化。 方法比较有或无环丙沙星诱导时嗜麦芽寡养单胞菌H407和 A26的MIC和EOP值，对在亚抑菌浓度环丙沙星中培育后的 菌株smeD基因进行RT-PCR扩增，监测表达水平。结果对抗 生素敏感的H407和A26泵抑制呈阳性或阴性反应。2株菌经 环丙沙星过夜孵育后测得的对氯霉素和环丙沙星MIC值比正 常非诱导时的MIC值高1?3个稀释度;加泵抑制剂CCCP后， 2个诱导株的MIC值均有明显下降。H407和A26经环丙沙星 诱导后氯霉素的EOP分别有和近4倍的增长。2株菌在抗菌 药物过夜和指数期添加生长时smeD的mRNA明显高于无抗菌 药物培养株。结论2株嗜麦芽寡养单胞菌临床株在环丙沙星 诱导下对部分抗菌药物的耐药性提高，与SmeDEF栗的诱导 表达增加有关。 关键词】嗜麦芽寡养单胞菌；外排泵；耐药性；诱 ABSTRACT Objective Toinvestigatec ABSTRACT Object ive Toinvestigatec hangesofantibiotic nicalisolatesof,anresistanceofthecli hangesofantibiotic nicalisolatesof,an resistanceofthecli dtherepressionleve lofeffluxpumpSmeDE ntibiotics. Methodstanceprofileofthe gardilut ionandthee OP)toantimicrobes, opumpinhibitorCCCPhemRNAlevelofsmeDbnceornotofciprofloantibiotic-sensiti lofeffluxpumpSmeDE ntibiotics. Method stanceprofileofthe gardilut ionandthee OP)toantimicrobes, opumpinhibitorCCCP hemRNAlevelofsmeDb nceornotofciproflo antibiotic-sensiti 6werepos i t iveandne torrespectively. MI ndchloramphenicoli Fbytheinductionofa s Toexaminetheresi clinicalstrainsbyafficiencyofplate(EandthEirreactionst ,andtoinvestigatetyRT-PCR,inthepresexacin. Results The vestrainsH407andA2 gativetopumpinhibi Csofciprofloxacinanducedbyciprofloxa cinwithsub-inhibit ionlevelwerehigher byl 3dilutionsthan thosewithoutinduct ion. Theincre-asesw ereinhibitedbyCCCP.Afterinductionbyc ereinhibitedbyCCCP .Afterinductionbyc iprofloxacin,theEOinH407andA26increa iprofloxacin,theEO inH407andA26increa esrespectively,and eDinthe2antimicrob seaswell. Conclusi severalantimicrobe fcouldbeinducedbyc Ptochloramphenicol sedbyandnearly4tim thelevelofmRNAofsm e-inducedstrainsro on Theresistanceto sinthetwoisolateso iprofloxacin. Thepo ssibleantibiotieresistancemechanisms ssibleantibiotiere sistancemechanisms seemedlikelybythem volvementoftheincr easedtranscription levelofsmeDEF. KEY WORDS Stenotrophom onasmaltophilia； A ntibioticresistanc e； Effluxpump； Ind uction 嗜麦芽寡养单胞