硅胶修饰-表面分子印迹牛血红蛋白及其识别性能的研究Studyof.PDF

2008 年第 66 卷 化 学 学 报 Vol. 66, 2008 第 1 期, 56～62 ACTA CHIMICA SINICA No. 1, 56 ～62 ·研究论文· 硅胶修饰-表面分子印迹牛血红蛋白及其识别性能的研究 刘秋叶a 李文友a 何锡文*,a 陈朗星a 张玉奎*,a,b (a 南开大学化学学院 天津 300071) (b 中国科学院大连化学物理研究所 大连 116011) 摘要 选用马来酸酐修饰的硅胶作为载体, 丙烯酰胺为功能单体, N,N'-亚甲基双丙烯酰胺为交联剂, 牛血红蛋白为模 板分子, 采用氧化还原悬浮聚合法, 合成了具有选择性识别的牛血红蛋白分子印迹聚合物. 并用红外光谱(IR)、扫描电 子显微镜(SEM)和元素分析对聚合物进行了表征, 结果表明载体表面成功地接枝了分子印迹聚合物薄层. 同时选择性 吸附实验表明分子印迹聚合物具有良好的识别性能, 能实现水溶液中牛血红蛋白的富集. 关键词 表面修饰分子印迹聚合物; 牛血红蛋白; 硅胶接枝; 氧化还原聚合 Study of Recognition Property of Molecularly Imprinted Bovine Hemoglobin on Surface-modified Silica Gel LIU, Qiu-Yea LI, Wen-Youa HE, Xi-Wen*,a CHEN, Lang-Xinga ZHANG, Yu-Kui*,a,b a ( Department of Chemistry, Nankai University, Tianjin 300071) (b Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011) Abstract The silica modified with maleic anhydride was synthesized as a carrier material. Then, molecu- larly imprinted polymers based on the surface-modified silica gel were prepared by redox polymerization of acrylamide with N,N'-methylene bisacrylamide as the functional monomer and the crosslinker in the pres- ence of bovine hemoglobin as a template molecule. The results of IR and scanning electron microscopy demonstrated the formation of a grafted polymer layer on the modified silica gel surface. The adsorption stu