溃疡性结肠炎小鼠结肠组织中ERK―1sRAGE表达的研究.docVIP

溃疡性结肠炎小鼠结肠组织中ERK―1sRAGE表达的研究 　　[摘要] 目的 探?溃疡性结肠炎小鼠结肠组织中细胞外调节蛋白激酶-1（ERK-1）、人可溶性晚期糖基化终末产物受体（sRAGE）的表达及意义。 方法 选取90只健康成年雄性BALB/c小鼠，根据随机数字表分为溃疡性结肠炎组（n=30）、细菌性肠炎组（n=30）及对照组（n=30），溃疡性结肠炎组给予饮用葡聚糖硫酸钠（DSS）造模，细菌性肠炎组给予大肠埃希菌菌液灌肠造模，对照组不做处理。造模成功后处死小鼠，观察各组小鼠疾病活动指数（DAI）、结肠组织病理学评分（HPS）；取小鼠结肠标本，应用免疫组化法测定ERK-1、sRAGE表达。 结果 与对照组、细菌性肠炎组相比，溃疡性结肠炎组小鼠DAI、HPS评分显著升高（P 0.05），而细菌性肠炎组DAI、HPS评分高于对照组（P 0.05）。溃疡性结肠炎组小鼠结肠组织中ERK-1、sRAGE阳性表达率均高于细菌性肠炎组及对照组（P 0.05），而细菌性肠炎组小鼠结肠组织中ERK-1表达高于对照组（P 0.05）。经Pearson相关分析显示，结肠组织中ERK-1、sRAGE与DAI、HPS评分呈正相关（P 0.05）。 结论 结肠组织中ERK-1、sRAGE异常表达与溃疡性结肠炎的发生及病情进展有密切关系。 　　[关键词] 细胞外调节蛋白激酶-1；人可溶性晚期糖基化终末产物受体；溃疡性结肠炎 　　[中图分类号] R574.62 [文献标识码] A [文章编号] 1673-7210（2016）11（c）-0008-04 　　The expression of ERK-1， sRAGE in colon tissue of mice with ulcerative colitis 　　YANG Jun CAO Yong WANG Dongxu PAN Di ZHANG Ying ZHENG Changqing 　　The Second Department of Digestive Internal Medicine， Shengjing Hospital Affiliated to China Medical University， Liaoning Province， Shenyang 117004， China 　　[Abstract] Objective To investigate the expression and significance of extracellular signal-regulated kinase-1 （ERK-1）， human soluble advanced glycation end product receptor （sRAGE） in colon tissue of mice with ulcerative colitis. Methods 90 healthy adult male BALB/c mice were selected and divided into ulcerative colitis group （n=30）， bacterial enteritis group （n=30） and control group （n=30） according to random number table. The rats in the ulcerative colitis group were given dextran sulfate sodium sulfate （DSS）， and the bacterial enteritis group was given E. coli bacteria liquid enema modeling. The control group had no dispose. The mice disease activity index （DAI）， colonic histopathology score （HPS） of each groups after the modeling were observed. The levels of ERK-1， sRAGE expression in assay colon tissue were determined by immunohistochemistry. Results The DAI， HPS scores of the ulcerative colitis group were higher than those of the bacterial enteritis group and the control group （P 　　[Ke