离体大鼠肠道菌对6种皂苷类成分代谢的研究.docVIP

离体大鼠肠道菌对6种皂苷类成分代谢的研究 　　[摘要] 为考察离体大鼠肠道菌群对6种皂苷类成分代谢转化的情况，将6种皂苷类成分单体（三七皂苷R1，人参皂苷Rg1，人参皂苷Rg2，人参皂苷Re，人参皂苷Rd和人参皂苷Rb1）分别与离体大鼠肠道菌群在厌氧条件下孵育8，24 h，通过乙腈沉淀蛋白和乙酸乙酯萃取处理后，采用LCQTOFMS/MS对代谢产物进行定性分析。通过比较空白对照样品和加药孵育样品的总离子流以及各个色谱峰的准分子离子和多级碎片离子，分析各皂苷类成分在大鼠粪便中可能的代谢产物。结果发现，大鼠肠道菌群对6种皂苷类成分具有显著的代谢转化作用，三七皂苷R1主要被代谢为5个产物，代谢途径为三七皂苷R1→人参皂苷Rg1→人参皂苷Rh1和人参皂苷F1→原人参三醇→脱氢原人参三醇；人参皂苷Rg1主要被代谢为4个产物，代谢途径为人参皂苷Rg1→人参皂苷Rh1和人参皂苷F1→原人参三醇→脱氢原人参三醇；人参皂苷Rg2主要被代谢为2个产物，代谢途径为人参皂苷Rg2→原人参三醇→脱氢原人参三醇；人参皂苷Re主要被代谢为4个产物，代谢途径为人参皂苷Re→人参皂苷Rg2→人参皂苷F1→原人参三醇→脱氢原人参三醇；人参皂苷Rd主要被代谢为4个产物，代谢途径为人参皂苷Rd→人参皂苷Rg3和人参皂苷F2→人参皂苷Rh2→原人参二醇；人参皂苷Rb1主要被代谢为5个产物，分别为人参皂苷Rb1→人参皂苷Rd→人参皂苷Rg3和人参皂苷F2→人参皂苷Rh2→原人参二醇。综上所述，6种皂苷类单体均能够被离体大鼠肠道菌群代谢，主要的代谢途径为脱糖基和脱氢。 　　[关键词] 大鼠肠道菌群； 皂苷类成分； LCQTOFMS/MS； 代谢； 脱糖基 　　Metabolism of six saponins by rat intestinal bacteria in vitro 　　GAO Xia1，2， GENG Ting1，2， MA Yang1，2， LI Yanjing1，2， HUANG Wenzhe1，2， WANG Zhenzhong1，2， XIAO Wei1，2* 　　（1Jiangsu Kanion Pharmaceutical Co.， Ltd.， Lianyungang 222001， China； 　　2State Key Laboratory of Pharmaceutical Newtech for Chinese Medicine， Lianyungang 222001， China） 　　[Abstract] To investigate the metabolism of six saponins by rat intestinal bacteria in vitroSix saponins， including notoginsenoside R1， ginsenoside Rg1， ginsenoside Rg2， ginsenoside Re， ginsenoside Rd and ginsenoside Rb1， were incubated for 8 and 24 h with rat intestinal bacteria under anaerobic environment， respectively After the samples were precipitated by acetonitrile and extracted with ethyl acetate， LCQTOFMS/MS was applied for the qualitative analysis of the metabolites The potential metabolites in rat feces were analyzed by comparing the total ion current of the test samples and blank samples and analyzing the quasimolecular ion and fragment ion of all chromatograms The results showed that six saponins could be easily metabolized by rat intestinal bacteria Notoginsenoside R1 was mainly metabolized into five metabolites， and it′s metabolic pathway was notoginsenoside R1→ginsenoside Rg1→ginsenoside Rh1 and ginsenosi