抗肿瘤化合物告达亭的早期毒性机制研究.docVIP

抗肿瘤化合物告达亭的早期毒性机制研究 为探究告达亭(Caudatin)对雄性昆明小鼠的毒性影响，按 照告达亭质量比为5和10 mg/kg以及溶剂对照组对SPF级昆明鼠通过 腹腔注射的方法，进行为期14 d的毒理学研究.实验动物总共30只昆 明鼠，随机分为6组，分2批进行实验，两次实验结果一致：实验昆 明鼠在不同浓度告达亭处理14 d后体质量无明显变化，血液学指标检 测正常，心、肝、肺、肾以及睾丸均无组织畸变. 和著作权归原 所有， 己 关键词告达亭；慢性毒理实验；血液成分生化分析 R99 A 1000-2537 (2017) 03-0032-05 Study on the Early Toxicity and Mechanism for Antitumor Compound Caudatin TANG Hong-yanl, HU Si-yang 1, 3, ZHANG Jia-wen 1, SHI Jia-yul， WU Xiao-yul， ZHANG Shanl, ZHANG Xiao-hanl，PENG Ke-hong2*，ZHOU Change (1. The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Science, Hunan Normal University, Changsha 410081, China； Department of Gynecological Oncology， Hunan Cancer Hospital, Changsha 410013， China； The High School Attached to HNU-Meixihu High School, Changsha 410205， China) Abstract To examine the toxic effect of Caudatin on male Kunming mice， using Caudatin concentrations of 5 mg/kg， 10 mg/kg and the vehicle control group of SPF grade Kunming mice by intraperitoneal injection method ， we conducted the toxicological study for a period of 14 days. These 30 Kunming mice were randomly divided into six groups ， within two sub-experiments. We found that the results from these two sub-experiments are consistent with each other. There was no significant change in body weight and hematological parameters. Wc observed no markedly pathological change in the tissue of heart, liver, lung, kidney and testis of 30 Kunming mice after the treatment of different concentrations of Caudatin for 14 days. Key words Caudatin； chronic toxicity test； biochemical analysis of blood components 近年来，中药新品种逐年增加，其应用的范围越来越广.关于中药 毒副作用给人体造成严重损害的报道时有发生，中药的安全性成为其 走向国际市场必须突破的瓶颈.C21留体苷类化合物主要存在于萝?科 中，告达亭(Caudatin)是从白首乌中分离得到的一种C21甾体苷元， 分子式为C28H4207, 其结构式如图1[1_2].前期研宄发现告达亭可以体外阻滞包括 AGS细胞和HcLa细胞在内的 多种肿瘤细胞周期，起到抑制细胞增殖的作用；通过克隆形成和 Tunel等实验证明告达亭可以诱导肿瘤细胞发生凋亡；划痕实验验证 告达亭对多种肿瘤细胞的迁移速率有显著的抑制作用[3-5].告达亭在 抗肿瘤、免疫调节、化疗药物增效减毒等方面具有重要的生物活性[6]. 但是到目前为止，未见有关告达亭药物毒性的报道.中药有效成分在开 发成新药前对其有效性及安全性做出